A kind of sheep oocyte in vitro "two-stage" maturation method and its pre-incubation solution and kit
An oocyte and in vitro maturation technology, applied in the field of embryo engineering, can solve problems affecting the production efficiency of in vitro embryos, it is difficult to achieve simultaneous nucleoplasmic maturation, and the impact and application of sheep oocytes are unknown, so as to improve in vitro development ability, The effect of improving in vitro development ability and good application prospect
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[0064] Experimental example, specific operation and effect verification of in vitro maturation of sheep oocyte of the present invention
[0065] 1. Mature culture of sheep oocytes
[0066] Sheep oocytes undergo conventional in vitro maturation and in vitro "two-stage" maturation provided by the present invention, the steps are as follows:
[0067] 1) Conventional in vitro maturation:
[0068] Step 1. Oocyte collection: use a sterile 10 mL syringe12 # The needle extracts follicles with a diameter of 3-6 mm from the surface of the ovary. Before collecting eggs, 1-2 mL of egg-collecting liquid should be extracted in a syringe, and cumulus eggs with uniform cytoplasm and relatively complete cumulus cells should be selected under a stereo microscope. Mother cell complexes (Cumulus Oocyte Complexes, COCs) were used for in vitro maturation (In Vitro Maturation, IVM).
[0069] Step 2. Oocyte maturation culture: select the cumulus oocyte complex with uniform cytoplasm and relatively...
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