Purely artificial feed suitable for breeding and experimental analysis of tea geometrid larvae
A technology of artificial feed and tea geometrid, applied in animal feed, animal husbandry, etc., can solve the problems of decreased survival rate, lack of tea powder, complex components of tea powder, etc., and achieve relatively clear, simple ingredients and good effect
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Embodiment 1
[0032] The improvement process for the tea geometrid pure artificial feed is divided into several stages. First, the inventor found that after casein was replaced by soybean powder, the growth of the tea geometrid was significantly improved; then the inventor replaced and increased the trace elements in the formula, and the improvement process is as follows:
[0033] Prepare the tea geometrid larvae feed respectively, adopt the formula in 201510116783.0 (the formula 5 with the highest survival rate in Table 1, hereinafter referred to as "Zhang Yongan's original feed" or "the original feed") and the preparation method of the improved feed are as follows:
[0034] Original feed formula: crush the wheat germ and pass through a 80-120 mesh sieve, weigh the agar in proportion, add water in proportion, boil for 1-2 minutes, then add wheat germ powder and casein in proportion, stir evenly and Boil until boiling, cool to 50-60°C; add Webster's salt, sorbic acid, methylparaben and inos...
Embodiment 2
[0044] Although the feeding effect of Example 1 is obviously improved, the effect of the tea geometrid artificial diet still cannot reach expectations. The inventor unexpectedly discovered that adding EGCG to the feed significantly improved the feeding effect of tea geometrid larvae, and could achieve the purpose of continuously supplying a large number of tea geometrids necessary for experimental treatment throughout the year.
[0045]Epigallocatechin gallate (abbreviated as EGCG), a main additive in the pure artificial feed for tea geometrid larvae in this example, is a catechin monomer isolated from tea leaves, and is the biological activity of tea polyphenols. main ingredient. EGCG is used in the food industry for anti-oxidation, preservation, and deodorization, in the pharmaceutical industry for antibacterial, anti-cancer, and anti-aging, and as a special functional additive in daily chemical products. However, in the insect kingdom, polyphenols are generally toxic to in...
Embodiment 3
[0056] 90 newly hatched first-instar larvae of the same batch of tea geometrid were selected and reared in a climate chamber with a temperature of (23±1)°C, a relative humidity of 75±5%, and a photoperiod of L:D=16:8. Insert the newly hatched larvae into the insect-raising box (30 heads per box), feed the artificial feed described in formula 3, and replace it with new feed every 2 to 3 days until the larvae pupate. Pupae were weighed and transferred to jars containing sand and kept moist with an appropriate amount of water until eclosion.
[0057] The pupation rate of larvae fed with this formula was 84.6%, the duration of larvae was 22.3 days, the average pupal weight was 101.6 mg, and the eclosion rate was 92.3%.
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