Nutrient slow-release filler loaded with functional microbes and application of filler

A microbial and functional technology used in the field of biological purification of environmental pollution

Active Publication Date: 2018-06-29
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a nutrient slow-release filler loaded with functional microorganisms and its application, which solves the problem that the existing filler needs to be in

Method used

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  • Nutrient slow-release filler loaded with functional microbes and application of filler
  • Nutrient slow-release filler loaded with functional microbes and application of filler
  • Nutrient slow-release filler loaded with functional microbes and application of filler

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1: packing support preparation process

[0039] (1) Filler carrier

[0040] At room temperature (25-30°C), slowly add 9g of sodium alginate powder into 600mL of inorganic salt culture solution under high-speed stirring at 160rpm, and continue stirring for 2 hours to obtain a colorless and transparent 15g / L sodium alginate solution mixed with inorganic nutrients. Then 50mL of sodium alginate solution was divided into 100mL beakers, and placed in a 4°C freezer for 16h until it became viscous. Spray 0.2mol·L after taking it out -1 CaCl 2 The aqueous solution a forms a film on its surface ( Figure 4 middle a), and then slowly add 0.2mol L by glass rod drainage -1 CaCl 2 Aqueous solution b, CaCl 2 Aqueous solution a and CaCl 2 The total volume of the aqueous solution b was 20mL, and the alginate gel was obtained after standing at 4°C for 72 hours, and finally freeze-dried at -81°C for 60 hours to form a porous structure and obtain a filler scaffold with a ...

Embodiment 2

[0046] Example 2: CaCl 2 Join method optimization

[0047] The preparation method of conventional calcium alginate gel is to adopt one-step method, promptly under normal temperature or low temperature 50ml sodium alginate solution (same as embodiment 1) directly mixes 20mL, 0.2mol L -1 CaCl 2 The aqueous solution was mixed, and the alginate gel was obtained after standing at 4°C for freezing reaction for 72 hours, and finally freeze-dried at -81°C for 60 hours to prepare a calcium alginate gel with a porous structure. However, the gel prepared by this method is often porous, which is not suitable for the transfer of nutrients and the growth of microorganisms.

Embodiment 3

[0048] Example 3: Evaluation of slow release performance of filler carrier with nutrient slow release function

[0049] (1) Filler carrier

[0050] The filler support with nutrient slow release function is prepared by the principle of emulsification and crosslinking. The preparation process is as follows: at room temperature, slowly add 18g of sodium alginate powder to 600mL of inorganic salt culture medium under high-speed stirring at 160rpm, and continue stirring for 2 hours to obtain a colorless Transparent 30g / L sodium alginate solution mixed with inorganic nutrients. Then 50mL sodium alginate solution was divided into 100mL beakers, and placed in a 4°C freezer for 24h until it became viscous. After taking it out, spray 0.1mol L on its surface -1 CaCl 2 Aqueous solution a forms a thin film, and then slowly add 0.1mol L -1 CaCl 2 Aqueous solution b, CaCl 2 Aqueous solution a and CaCl 2 The total volume of the aqueous solution b was 25 mL, and the alginate gel was obt...

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Abstract

The invention discloses nutrient slow-release filler loaded with functional microbes and an application of the filler. The filler is prepared from porous alginate gel which serves as a support and isloaded with microbes. It is found that the prepared nutrient slow-release filler loaded with the functional microbes can slowly release nutrients to adjust environment pH during usage after being putinto a reactor to operate, addition of an inorganic nutrient solution and alkali liquor adjustment are not required, and the degradation efficiency for butyl acetate and the like is higher than that by a contrast reactor with common polyurethane foam filler. Meanwhile, the filler can significantly shorten the start period of the reactor and plays very important roles in the waste gas biological purification field.

Description

(1) Technical field [0001] The invention belongs to the technical field of biological purification of environmental pollution, and relates to the preparation method of the nutrient slow-release filler loaded with functional microorganisms and its application in waste gas biological purification facilities. (2) Background technology [0002] In recent years, the biological purification method is a method of exhaust gas purification with great potential, and its research is very popular in various countries and regions. Filler is an important part of the biological purification system, and its performance directly affects the removal efficiency of the entire purification system. Therefore, it is necessary to carry out research on the characteristics of fillers and develop new biological fillers for waste gas treatment. [0003] At present, the fillers used in biological purification systems mainly include natural fillers and organic synthetic fillers. Among them, natural fill...

Claims

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Application Information

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IPC IPC(8): C12N11/10B01D53/85
CPCB01D53/85B01D2257/91C12N11/10Y02A50/20
Inventor 成卓韦陈建孟於建明陈东之叶杰旭徐丹华
Owner ZHEJIANG UNIV OF TECH
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