Blood sample density separation liquid and preparation method thereof

A technology for density separation and blood samples, which is applied in the field of cell separation and detection, can solve the problems of single component and low permeability of density separation liquid, and achieve the effects of easy aggregation and sedimentation, multiple components, and improved efficiency

Inactive Publication Date: 2018-07-03
孝感市森茂激光数控设备有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The existing density separation fluid has a relatively single component. Commonly used media include sucrose, serum albumin, polysucrose, cesium chloride, etc. Although the effect is good,

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] A blood sample density separation solution, the main component is double distilled water, its specific composition is: 55g / L glucose, 110g / L diatrizoate meglumine and 12g / L ethanol, and the pH value of the solution is 7.3±0.1. The preparation method of the separation liquid is: accurately weigh 5.5g of glucose and 11g of meglumine diatrizoate, put the weighed raw materials into a beaker, add 50mL of double-distilled water into the beaker and stir, and slightly heat the beaker to accelerate solid dissolution. When no solid can be seen at the bottom of the beaker, weigh 1.2g of absolute ethanol and add it to the mixed solution, continue to add double distilled water to make up the volume of the solution to 100mL, and finally add NaOH solution with a concentration of 1.5mol / L to the solution to adjust the pH to 7.3±0.1. The whole preparation process must be carried out in a clean and pollution-free environment, and the prepared separation liquid can be stored in a refriger...

Embodiment 2

[0016] A blood sample density separation solution, the main component is twice distilled water, its specific composition is: 85g / L glucose, 92g / L diatrizoate and 14g / L ethanol, and the pH value of the solution is 7.3±0.1. The preparation method of the separating liquid is as follows: accurately weigh 85g of glucose and 92g of meglumine diatrizoate, put the weighed raw materials into a beaker, add 600mL of double-distilled water into the beaker and stir, and slightly heat the beaker to accelerate solid dissolution. When no solid can be seen at the bottom of the beaker, weigh 14g of absolute ethanol and add it to the mixed solution, continue to add twice distilled water to make up the volume of the solution to 1000mL, and finally add NaOH solution with a concentration of 1.5mol / L to the solution to adjust the pH to 7.3±0.1 is obtained.

Embodiment 3

[0018] A blood sample density separation solution, the main component is double distilled water, its specific composition is: 80g / L glucose, 100g / L diatrizoate meglumine and 10g / L ethanol, and the pH value of the solution is 7.3±0.1. The preparation method of the separating liquid is as follows: accurately weigh 80 g of glucose and 100 g of meglumine diatrizoate, put the weighed raw materials into a beaker, add 500 mL of double distilled water into the beaker and stir, and slightly heat the beaker to accelerate solid dissolution. When no solid can be seen at the bottom of the beaker, weigh 10g of absolute ethanol and add it to the mixed solution, continue to add twice distilled water to make up the volume of the solution to 1000mL, and finally add NaOH solution with a concentration of 1mol / L to the solution to adjust the pH to 7.3 ±0.1 that is.

[0019] The density separation liquid prepared by the present invention is stored in a container and refrigerated. Before use, wait f...

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Abstract

The invention relates to multi-component blood sample density separation liquid with excellent comprehensive performance and a preparation method thereof. The density separation liquid is prepared bythe steps: by taking glucose and meglumine diatrizoate as main ingredients, adding a small amount of NaOH and ethanol, and preparing a mixed solution. The specific composition is as follows: the content of glucose is 50-90 g/L, the content of the meglumine diatrizoate is 90-140 g/L, the ethanol content is 10-15 g/L, and the pH value of the solution is 7.3+/-0.1. With the adoption of the separationliquid, blood cells with enough quantity can be quickly settled and separated, and effects of foreign similar imported products can be achieved.

Description

technical field [0001] The invention relates to the technical field of cell separation and detection, in particular to a blood sample separation liquid and a preparation method thereof. Background technique [0002] The most commonly used method for separating biological particle mixtures (such as blood) is density gradient centrifugation, which is one of the common methods for cell separation and purification based on cell density differences, with the help of cell separation fluid and centrifugal force. Density gradient centrifugation produces a certain degree of density gradient through the sample density separation medium, and the diluted blood is layered on the separation medium. The specificity of red blood cells and granulocytes is large (1.090g / mL), and they sink to the bottom of the tube after centrifugation; The specific gravity of cells and monocytes (1.075-1.090g / mL) is less than or equal to that of the layered liquid, and they float on the liquid surface of the ...

Claims

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Application Information

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IPC IPC(8): C12N5/078
CPCC12N5/0634C12N2509/00
Inventor 孙红安
Owner 孝感市森茂激光数控设备有限公司
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