Extraction method of exosomes from mouse brain slices

An extraction method and exosome technology, applied in biochemical equipment and methods, nervous system cells, microorganisms, etc., can solve the problem of exosome extraction method without brain tissue release, difficulty in extracting mouse cerebrospinal fluid, and exosome concentration Low-level problems, to achieve the effects of saving experimental costs, improving computing efficiency, and using less

Active Publication Date: 2021-04-06
XUZHOU MEDICAL UNIV
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Problems solved by technology

[0004] The method of cell culture is used to collect exosomes, the amount of medium used is large, and the concentration of collected exosomes is low, which wastes experimental reagents; for brain science research, cerebrospinal fluid can also be studied, but it is difficult to extract mouse cerebrospinal fluid and cannot Achieved; currently there is no extraction method for exosomes released from brain tissue

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  • Extraction method of exosomes from mouse brain slices
  • Extraction method of exosomes from mouse brain slices
  • Extraction method of exosomes from mouse brain slices

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[0017] In order to make the above objects, features and advantages of the present invention more obvious and comprehensible, specific implementations of the present invention will be described in detail below in conjunction with the accompanying drawings.

[0018] In the following description, a lot of specific details are set forth in order to fully understand the present invention, but the present invention can also be implemented in other ways different from those described here, and those skilled in the art can do it without departing from the meaning of the present invention. By analogy, the present invention is therefore not limited to the specific examples disclosed below.

[0019] Second, "one embodiment" or "an embodiment" referred to herein refers to a specific feature, structure or characteristic that may be included in at least one implementation of the present invention. "In one embodiment" appearing in different places in this specification does not all refer to ...

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Abstract

The invention provides a method for extracting exosomes from mouse brain slices. The method is to collect and extract exosomes in the culture medium by culturing mouse brain slices, so as to obtain exosomes for subsequent experiments. sample. This method provides a new exosome collection method, combined with the exosome extraction kit, optimizes the current exosome extraction method, enables the extraction of exosomes in brain tissue, and fills the technical gap. This method is easy to operate, saves the cost of the experiment, does not have high requirements on the skills of the experimenters and experimental equipment, has no effect on the downstream experiments, and the experimental results are safe and reliable.

Description

technical field [0001] The invention relates to a method for collecting and extracting exosomes released from tissues, in particular to a method for extracting exosomes from mouse brain slices, and belongs to the field of biotechnology. Background technique [0002] Exosomes are small membrane vesicles that can be secreted by most cells. They have a lipid bilayer membrane structure with a diameter of 30-100nm. Cell-specific proteins are contained in these tiny membrane vesicles. , nucleic acid and other substances with genetic information. Existing collection and extraction methods for exosomes are generally directly extracted from body fluids, and some cells are cultured for specific cells and collected and extracted from the culture medium. These methods have certain limitations, and exosomes cannot be extracted from local tissues. Brain slice culture is an organ culture method developed in recent years. Compared with traditional cell culture, brain slice culture retain...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/079
CPCC12N5/0618C12N2500/12C12N2500/14C12N2500/16C12N2500/34
Inventor 喻晓路郭健鹏
Owner XUZHOU MEDICAL UNIV
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