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The miRNA markers related to pig carcass lean meat percentage and its application

A technology of markers and lean meat percentage, applied in the field of miRNA markers

Active Publication Date: 2021-05-28
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In fact, the final occurrence of the trait of high lean meat in improved pig breeds is a complex physiological and biochemical process affected by multiple factors such as genetics, environment, and nutritional levels; The effect of changing the genetic background of local pig breeds in China is unmatched by factors such as environment and nutritional levels

Method used

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  • The miRNA markers related to pig carcass lean meat percentage and its application
  • The miRNA markers related to pig carcass lean meat percentage and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 High-throughput miRNA-seq identification of differentially expressed miRNAs in different breeds of pig muscle tissue

[0038] 1) Landrace pigs with large differences in carcass lean meat percentage and Lantang pigs, a special breed of Guangdong, were selected as the research objects; 3 cases of Landrace pigs and 3 cases of Lantang pigs were selected to obtain their back muscle tissues, and the tissue samples were quickly frozen after they were taken out. In liquid nitrogen for 30 seconds, then stored in a -80°C refrigerator, and then sent to a sequencing company with dry ice for small RNA sequencing.

[0039] 2) Use FastQC (http: / / www.bioinformatics.babraham.ac.uk / projects / fastqc / ) software to evaluate the overall quality of sequencing data, including base quality value distribution, quality value position distribution, GC content, PCR duplication content, etc.

[0040] After high-throughput sequencing, the total number of reads obtained from the three back m...

Embodiment 2

[0044] Example 2 Detection of ssc-miR-423-5p expression in porcine muscle tissue by fluorescent quantitative PCR

[0045] Reagent:

[0046] Reverse transcription kit: PrimeScript TM RT reagent Kit with gDNA Eraser (Perfect RealTime), #RR047Q, Takara;

[0047] Quantitative PCR reagents: Fast qPCR Mix, #RR430A, Takara.

[0048] Relevant experimental items to RNase treatment:

[0049] 1) Rinse and soak all glassware with DEPC before use, high pressure at 120°C for 20 minutes, and dry at high temperature at 180°C for more than 2 hours.

[0050] 2) Soak plastic utensils (such as EP tubes / tips) in 0.1% DEPC water overnight before use, and then dry the liquid, 120°C and high pressure for 20 minutes, and dry in an oven for later use.

[0051] Total RNA extraction:

[0052] Select 50 cases of Landrace pig muscle tissue and 50 cases of Lantang pig muscle tissue, and use the TRIzol method according to the instructions of the Invitrogen kit. The specific steps are as follows:

[0...

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Abstract

The invention discloses a miRNA marker related to pig carcass lean meat percentage and application thereof, and belongs to the technical field of livestock molecular marker preparation. The miRNA marker is ssc-miR-423-5p, the sequence of which is shown in SEQ ID NO:1. The ssc-miR-423-5p regulates pig muscle development by combining with its target gene GH. It is low-expressed in lean muscle tissue, highly expressed in fat-type muscle tissue and regulates the low expression of target gene GH. It is applied to pig muscle development assisted genetic selection process. The ssc-miR-423-5p disclosed in the present invention can be used as a molecular marker related to pig lean meat percentage, and has important practical application value in genetic assisted breeding.

Description

technical field [0001] The invention belongs to the technical field of livestock molecular marker preparation, and in particular relates to a miRNA marker related to pig carcass lean meat percentage and application thereof. Background technique [0002] Animal muscle formation and quality traits improvement are the most important research directions in livestock breeding. In recent years, Chinese breeders have carried out a large number of breeding work of new pig breeds (lines) through the combination of modern molecular breeding technology and traditional breeding technology. Chinese local pig breeds. Since the last century, many regions in my country have carried out the selection and breeding of lean-meat pig breeds, in order to achieve the goal of breeding local lean-meat pig breeds suitable for my country's national conditions. In this process, the Landrace pig is a typical lean-meat pig among the current imported improved breeds, and almost all places use Landrace p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/113C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/158C12Q2600/178
Inventor 孙加节张永亮江青艳习欠云陈婷罗君谊
Owner SOUTH CHINA AGRI UNIV