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Biomedical application of triptonide to preparation of Gli gene inhibitor and liver cancer prevention and control drug

A technology of triptolide ketone and biomedicine, which is applied in the field of biomedicine for the preparation of Gli gene inhibitors and drugs for preventing and treating liver cancer, and can solve the problems that triptolide ketone has not been found to prevent and treat liver cancer.

Inactive Publication Date: 2018-07-24
赖旭宇
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There is no report on the prevention and treatment of liver cancer by triptolide

Method used

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  • Biomedical application of triptonide to preparation of Gli gene inhibitor and liver cancer prevention and control drug
  • Biomedical application of triptonide to preparation of Gli gene inhibitor and liver cancer prevention and control drug
  • Biomedical application of triptonide to preparation of Gli gene inhibitor and liver cancer prevention and control drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Effect of triptolide on the proliferation of human liver cancer HepG2 cells

[0023] 1. Experimental materials

[0024] Human liver cancer HepG2 cell line was purchased from Shanghai Jikai Gene Technology Co., Ltd.;

[0025] DMEM medium, fetal bovine serum and PBS were purchased from Gibco Company; TRIzol was purchased from Invitrogen Company; the reverse transcription kit was purchased from Fermentas Company of the United States, and the fluorescent quantitative PCR kit was purchased from TaKaRabao Bioengineering (Dalian) Co., Ltd.; cell lysate , SDS-PAGE protein loading buffer (5×), BCA protein concentration test kit, 20×TPBS buffer, etc. were purchased from Jiangsu Biyuntian Biotechnology Research Institute, PVDF membrane was purchased from Millipore, USA; CCK-8 kit Purchased from Tongren Institute of Chemistry in Japan; Gli1, Gli2 and GAPDH primers were synthesized by Shanghai Sangon Bioengineering Company;

[0026] Rabbit anti-human Gli1 monoclonal ant...

Embodiment 2

[0058] Example 2: Effect of triptolide on the proliferation of human liver cancer SMMC-7721 line cells

[0059] 1. Experimental materials

[0060] Human liver cancer SMMC-7721 cell line was purchased from Shanghai Jikai Gene Technology Co., Ltd.;

[0061] DMEM medium, fetal bovine serum and PBS were purchased from Gibco Company; TRIzol was purchased from Invitrogen Company; the reverse transcription kit was purchased from Fermentas Company of the United States, and the fluorescent quantitative PCR kit was purchased from TaKaRabao Bioengineering (Dalian) Co., Ltd.; cell lysate , SDS-PAGE protein loading buffer (5×), BCA protein concentration test kit, 20×TPBS buffer, etc. were purchased from Jiangsu Biyuntian Biotechnology Research Institute, PVDF membrane was purchased from Millipore, USA; CCK-8 kit Purchased from Tongren Institute of Chemistry in Japan; Gli1, Gli2 and GAPDH primers were synthesized by Shanghai Sangon Bioengineering Company;

[0062] Rabbit anti-human Gli1 m...

Embodiment 3

[0094] Example 3: Effect of triptolide on the growth of liver cancer xenografts in nude mice

[0095] HepG2 cells in a well-growth logarithmic growth phase were digested with trypsin, centrifuged and counted with trypan blue, and made 1×10 with normal saline 7 cells / mL of cell suspension were inoculated at 0.5 cm of the right axilla of nude mice, and each nude mouse was inoculated with 0.1 mL of cell suspension. Use a vernier caliper to measure the diameter of the transplanted tumor in nude mice, and wait until the tumor grows to about 100mm 3 Nude mice were randomly divided into triptolide low-dose group (12.5mg / kg / d), triptolide high-dose group (25mg / kg / d) and control group, 10 in each group. The administration plan of the triptolide group was as follows: intragastric administration, once a day, and the control group was given an equal volume of vehicle 0.5% CMC-Na. Administration for three weeks. Six hours after the last administration, the nude mice were sacrificed, and...

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Abstract

The invention discloses biomedical application of triptonide to preparation of a Gli gene inhibitor and a liver cancer prevention and control drug. The invention finds out that the triptonide can be used for remarkably inhibiting proliferation of liver cancer cells through inhibiting expression of Gli1 and Gli2 genes and the inhibition effect has remarkable dosage dependence; the triptonide is aneffective inhibitor of the Gli1 and Gli2 genes and can be used for preparing the liver cancer prevention and control drug. The technicians in the field know that the expression of the Gli1 and Gli2 genes is a relatively developed anti-cancer molecular pathway and the triptonide plays a role of resisting liver cancer through the pathway. Meanwhile, the triptonide is the effective inhibitor of the expression of the Gli1 and Gli2 genes; the triptonide also can be used for preparing drugs for treating diseases related to high expression of the Gli1 and Gli2 genes.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to the new application of known compounds, in particular to the biomedical application of triptolide in the preparation of Gli gene inhibitors and drugs for preventing and treating liver cancer. Background technique [0002] Triptolide ketone is a natural product isolated from Tripterygium wilfordii, and its chemical structural formula is as follows: [0003] [0004] Studies have shown that triptolide can reduce the expression of cancer-promoting non-coding RNA in gastric cancer cells, significantly inhibiting its malignant behavior, and has good application value in the treatment of gastric cancer (reference: triptolide inhibits gastric cancer Research on the role and mechanism of cell malignant behavior, Bulletin of Traditional Chinese Medicine, Volume 16, Issue 5, 2017). [0005] So far, no report has been found on triptolide in the prevention and treatment of liver cancer. When the...

Claims

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Application Information

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IPC IPC(8): A61K31/585A61P35/00A61P1/16
CPCA61K31/585
Inventor 赖旭宇陈世伟
Owner 赖旭宇
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