Methods, markers and kits for diagnosis, screening and risk prediction of colorectal cancer

A risk prediction and kit technology, applied in the field of disease detection, can solve problems such as psychological pain, poor compliance, and reduced willingness of subjects to be screened, and achieve the effect of low cost and convenient detection.

Active Publication Date: 2022-01-18
太科航天智能康养技术(深圳)有限责任公司
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Problems solved by technology

The conventional gold standard for the diagnosis of colorectal cancer is colonoscopy, but it is traumatic and psychologically painful, and patients generally resist it; fecal occult blood test requires multiple sampling, which will bring a lot of inconvenience to patients, resulting in the screening of patients. Decreased willingness to investigate and poor compliance

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  • Methods, markers and kits for diagnosis, screening and risk prediction of colorectal cancer
  • Methods, markers and kits for diagnosis, screening and risk prediction of colorectal cancer
  • Methods, markers and kits for diagnosis, screening and risk prediction of colorectal cancer

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Embodiment Construction

[0035] The human ribosomal protein S24 (RPS24) gene includes 6 exons, which encode ribosomal protein S24, which is a component of ribosomal protein 40s. In vitro cell studies have shown that the abnormal expression of RPS24 gene is related to the proliferation and metastasis of colorectal cancer cells, but the specific mechanism is still unclear. The present inventors have found that colorectal cancer is related to the methylation of the sequence before and after the transcription start point of the first exon of the gene RPS24. The inventor speculates that the gene RPS24 may regulate gene expression through DNA methylation, which is one of the important factors leading to the occurrence of colorectal cancer.

[0036] The present inventors have found that among the three groups of CpG in the sequence before and after the transcription initiation point of gene RPS24 as follows, group (1) and the combination of group (1) and group (2) or group (3): (1)+22, +27 and +29; (2) -24,...

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Abstract

The present invention discloses DNA methylation markers for diagnosis, screening and prediction of male colorectal cancer. The markers are group (1) or group (1) of the following three groups of CpG sites before and after the transcription start point of the gene RPS24 Group (1) methylated in combination with group (2) or group (3): +22, +27, and +29; (2)‑24, ‑18, +89, and +92; (3)+ 136, the sequence numbering is based on the numbering of the sequence shown in SEQ ID NO.1, wherein the A starting from the 143rd transcription start point is +1. The invention also discloses a probe, a method and a kit for detecting the DNA methylation marker, and a computer module for predicting the risk of male colorectal cancer by using the data of the DNA methylation site.

Description

technical field [0001] The present invention relates to the field of disease detection; more specifically, the present invention relates to a method, marker and kit for colorectal cancer diagnosis, screening and risk prediction through detection of DNA methylation in peripheral blood cells. Background technique [0002] The morbidity and mortality of colorectal cancer in my country rank fifth among all tumor morbidity and mortality respectively. For cancer, the significance of early screening far exceeds that of treatment. The conventional gold standard for the diagnosis of colorectal cancer is colonoscopy, but it is traumatic and psychologically painful, and patients generally resist it; fecal occult blood test requires multiple sampling, which will bring a lot of inconvenience to patients, resulting in the screening of patients. The willingness to investigate is reduced, and the compliance is poor. The hematological examination of cancer markers is non-invasive and conven...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/154
Inventor 吴安东刘长宁宁争志
Owner 太科航天智能康养技术(深圳)有限责任公司
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