Application method of prussian blue tracer agent in lymph node development

Abstract

The invention discloses an application method of a prussian blue tracer agent in lymph node development. The method comprises the steps that 24 young female New Zealand white mice, 24 young male New Zealand white mice, 24 adult female New Zealand white mice, 24 adult male New Zealand white mice, 24 old female New Zealand white mice and 24 old male New Zealand white mice are taken; all the mice areaveragely divided into a first experimental group, a second experimental group, a third experimental group, a first comparison group, a second comparison group and a third comparison group; an axillary reactive hyperplasia lymph node model and a breast cancer axillary metastasis lymph node model are established in the first experimental group, a brain tumor lymph node model and a brain tumor lymph node metastasis model are established in the second experimental group, a neck lymph node model and a neck lymph node metastasis model are established in the third experimental group. The method hasthe advantages that by inoculating male and female white mice of different ages against sarcomas, and by observing the lymph node metastasis through the prussian blue tracer agent, it is learnt thatthe prussian blue tracer agent can move along with the metastasis of the lymph node, so the prussian blue tracer agent can be used for medically determining whether the lymph node metastasizes or notafter an operation.

Description

technical field [0001] The invention relates to a Prussian blue tracer, in particular to an application method of the Prussian blue tracer in lymph node visualization. Background technique [0002] The Prussian blue tracer is ferricyanide, and the preparation method of ferricyanide: react with yellow blood salt potassium and ferrous sulfate in a slightly acidic solution to generate a double salt of ferrous ferrocyanide, and then add potassium chlorate to oxidize Generate the double salt of ferric ferrocyanide and potassium ferrocyanide; if yellow blood salt sodium is used to make iron blue, the generated double salt should be replaced by ammonium sodium, that is, ferric ferrocyanide and ferrous ferrocyanide will be generated. Ammonium ferricyanide double salt, so iron blue is divided into potassium iron blue and ammonium iron blue according to different raw materials; the above double salt is filtered, rinsed, dried, and crushed to obtain dark blue iron blue pigment. Prussi...

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Problems solved by technology

Prussian blue tracer is widely used and can be used as a fluorescent agent in medicine, but the application of Prussian blue tracer on lymph nodes is very scarce, which brings inconvenience to people

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