Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A nasal immune carrier based on antigen covalently bound to chitosan nanoparticles

A chitosan nanoparticle, covalent binding technology, applied in the field of medicine, can solve the problems of unsatisfactory immune effect, incomplete release, low encapsulation rate, etc., achieves good nasal cavity immune effect, enhances immune response, and has a simple preparation method Effect

Active Publication Date: 2016-08-17
FUDAN UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the chitosan nanoparticle is used as the main way of mucosal immune adjuvant to encapsulate the antigen inside the nanoparticle, but this method has obvious disadvantages: (1) the encapsulation efficiency is low; (3) The antigens that can contact APC and be recognized and presented by APC are less, making the immune effect unsatisfactory

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A nasal immune carrier based on antigen covalently bound to chitosan nanoparticles
  • A nasal immune carrier based on antigen covalently bound to chitosan nanoparticles
  • A nasal immune carrier based on antigen covalently bound to chitosan nanoparticles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Weigh 0.1 g of chitosan, dissolve it in 50 mL of 1% acetic acid, add 3-maleimido propionate hydroxysuccinimide solution dropwise under stirring, and react for 1 h. After the reaction, the reaction solution was dialyzed for 24 h and freeze-dried to obtain maleimide-chitosan (Mal-CS).

[0055] Weighed an appropriate amount of Mal-CS and dissolved it in sodium acetate buffer (pH5.5), added an equal volume of sodium tripolyphosphate solution, and stirred at room temperature for 30 min to prepare a blank nanoparticle suspension. The suspension was mixed with thiolated ovalbumin (OVA) and reacted at room temperature for 10 min to obtain chitosan nanoparticles covalently bound to OVA.

Embodiment 2

[0057] Weigh 2.5 g of chitosan, add to 250 mL of 1% acetic acid to dissolve. Add 0.10~0.14 g ammonium persulfate, fill with N 2 Protect, heat and stir, then add 1.2~2.0 g methacryloyloxyethyltrimethylammonium chloride (TMAEMC), and react for 2~5 h. The reaction solution was dialyzed for 24 h and freeze-dried to obtain quaternized chitosan (TMC).

[0058] Weigh 0.5 g of TMC, dissolve it in 50 mL of sodium acetate buffer solution, add 3-maleimidopropionic acid hydroxysuccinimide ester solution dropwise under stirring, and react for 1 h. After the reaction, the reaction solution was dialyzed for 24 h and freeze-dried to obtain maleimide-quaternized chitosan (Mal-TMC).

[0059] The prepared TMC, Mal-TMC were dissolved in deuterated acetic acid / heavy water, 1 H NMR identification of its structure, the results are as follows figure 1 shown; TMC showed a strong (-N + (CH 3 ) 3 ) peak, confirming that TMAEMC has been attached to chitosan. Mal-TMC has an obvious peak (maleimide...

Embodiment 3

[0061] Weighed an appropriate amount of Mal-TMC and dissolved it in HEPES buffer (pH 7.0), added an equal volume of sodium tripolyphosphate solution, and stirred at room temperature for 30 min to prepare a blank nanoparticle suspension. The suspension was mixed with thiolated ovalbumin (OVA), and reacted at room temperature for 10 min to obtain TMC nanoparticles covalently linked by OVA.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
particle diameteraaaaaaaaaa
particle sizeaaaaaaaaaa
Login to View More

Abstract

The invention belongs to the technical field of medicine, and relates to a nasal immune carrier based on an antigen covalently bound to chitosan nanoparticles and a preparation method thereof; the carrier is composed of nanoparticles and antigens, and the antigen is covalently bonded on the surface of the nanoparticles , wherein the nanoparticles are prepared from chitosan and its derivatives; the antigen is selected from proteins or polypeptides associated with infectious diseases or tumors; the carrier of the present invention can significantly improve the cervical lymph node target after nasal administration of the antigen protein or polypeptide. tropism, enhance its mucosal and systemic immune response; the carrier is simple to prepare, stable in process, high in antigen utilization, not easy to fall off, and conducive to improving the transport of antigens in cervical lymph nodes, and the carrier has good nasal immune effect and no nasal mucosal toxicity , suitable for the prevention of diseases transmitted by the respiratory tract.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to a nasal immune carrier based on antigen covalently binding chitosan nanoparticles and a preparation method thereof. Background technique [0002] In recent years, respiratory infectious diseases caused by bacteria, fungi, viruses, etc. have occurred frequently, such as the SARS outbreak in 2003, the H5N1 avian influenza outbreak in 2005, the H1N1 influenza pandemic in 2009, and the recent H7N9 new avian influenza, etc. , a serious threat to human health; therefore, how to prevent the spread of respiratory infectious diseases is still a difficult task for medical workers. At present, there is still a lack of effective large-scale air disinfection methods, so the focus of its prevention lies in the vaccination of corresponding vaccines. [0003] Respiratory tract infectious diseases are mainly caused by pathogens entering or infecting the respiratory mucosa, and the nasal mucosa is...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/385A61K39/39A61K47/48
CPCY02A50/30
Inventor 张奇志刘青锋郑晓瑶邵夏炎蒋新国
Owner FUDAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com