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Application of CD4+T lymphocyte characteristic protein to identification of latent tuberculosis infection and active tuberculosis

A technology for active pulmonary tuberculosis and lymphocytes, which can be applied in the biological field and can solve problems such as lack of clinical evidence

Active Publication Date: 2018-08-10
BEIJING CHEST HOSPITAL CAPITAL MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In addition, although there are new markers such as: interleukin-2 (IL-2), HSP16.3, interferon-inducible protein 10 (IP-10) and monocyte chemotactic protein 2 (MCP-2), etc. Emerging, but still lack sufficient clinical evidence

Method used

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  • Application of CD4+T lymphocyte characteristic protein to identification of latent tuberculosis infection and active tuberculosis
  • Application of CD4+T lymphocyte characteristic protein to identification of latent tuberculosis infection and active tuberculosis
  • Application of CD4+T lymphocyte characteristic protein to identification of latent tuberculosis infection and active tuberculosis

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Experimental program
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Embodiment 1

[0053] Embodiment 1, the CD4 that distinguishes active pulmonary tuberculosis and latent tuberculosis infection + Discovery and application of T lymphocyte characteristic protein

[0054] The instruments used in the following experiments are liquid chromatography-electrospray ionization tandem mass spectrometry (Q-Exactive ThermoFinnigan), electrophoresis instrument (Bio-rad, USA), and MultiSkans microplate reader (Thermo, USA).

[0055] 1. CD4 + Collection of T lymphocytes

[0056] Blood samples were collected from patients with clinically diagnosed LTBI, patients with newly diagnosed active pulmonary tuberculosis (to prevent drugs from affecting the body) and healthy controls, 9 cases in each. All blood samples were drawn in the morning on an empty stomach, and 5.0ml of peripheral blood was collected using a disposable vacuum anticoagulant tube.

[0057] Within 2 hours, use lymphocyte separation medium (Lymphoprep, Stemcell) to extract peripheral blood mononuclear cells (...

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Abstract

The invention discloses application of CD4+T lymphocyte characteristic protein to identification of active tuberculosis and latent tuberculosis infection (LTBI). The invention provides application ofa substance for detecting an expression quantity of integrin beta-3 and / or integrin-linked kinase to preparation of a product for distinguishing or identifying or assisting to distinguish or identifya patient with the active tuberculosis and a latent tuberculosis infection infected person. The invention finds out that integrin beta-3 and integrin-linked kinase protein can distinguish an LTBI patient and an initially-treated active tuberculosis patient, and novel diagnostic standards are provided for diagnosing the LTBI patient and the initially-treated active tuberculosis patient.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the application of CD4+T lymphocyte characteristic protein in identifying active pulmonary tuberculosis and latent tuberculosis infection. Background technique [0002] In 2015, the World Health Organization defined LTBI in the "Guidelines for the Diagnosis and Treatment of MTB (Mycobacterium tuberculosis, Mtb) Latent TB Infection (LTBI)" as no clinical manifestations of active tuberculosis, but the body continues to have immunity to MTB antigen stimulation. A state of response. According to the World Health Organization, about 1 / 3 of the people in the world are infected with Mycobacterium tuberculosis, of which more than 95% manifest as latent infection of tuberculosis, and 5-10% of those infected with LTBI will eventually develop into active tuberculosis and become the main cause of tuberculosis. source of infection. The large number of LTBI infections is the key and ...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/569
CPCG01N33/5695G01N33/6884G01N2333/35G01N2800/12
Inventor 黄银霞张宗德
Owner BEIJING CHEST HOSPITAL CAPITAL MEDICAL UNIV
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