Tissue culture mass production method adopting apple dwarfing rootstock M9T337

Abstract

The invention belongs to the technical field of plant tissue culture, and particularly relates to patent application matter of a new tissue culturing method of stable apple dwarfing rootstock M9T337.The culturing method comprises the main steps: performing culturing by mainly utilizing a primary generation of culture medium, a first type subculture medium, a second type subculture medium and a rooting culture medium. According to the application, growth hormone combination and concentration of the growth hormone combination in the culture medium are optimized, and meanwhile, through an optimal tissue culturing method, alternate use of different types of culture mediums in the subculture culture is combined, so that the phenomena of vitrification seedling and yellow seedling are effectively reduced, breeding coefficients are stabilized by 5 times, the seedling height is 3cm or more, and the proportion of breeding and rooting is controlled to 1 to (2 to 3); further, through combinationof rooting culture and seedling exercising, the transplanting time is shortened, so that about original 40 days are reduced to about 30 days from the rooting step to the transplanting step is realized, a total rooting ratio is stabilized to about 88 percent, a transplanting survival ratio reaches about 84 percent, and a better tissue culture production effect is achieved.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and specifically relates to a patent application for a new method for tissue culture of a stable apple dwarf stock M9T337. Background technique [0002] Apple is the largest fruit variety in my country, with a planting area of ​​about 37 million mu (2016). For a long time, the main planting mode of apples in my country is arbor anvil, large crown and sparse planting. There are problems such as tall tree body, low planting density, poor fruit commerciality, and cannot be operated mechanically, which is no longer suitable for the development of modern fruit industry. Dwarf rootstock close-planting cultivation has the advantages of small tree crown, early fruiting, good fruit quality, easy standardization and mechanized production, etc., and has become the development trend of apple cultivation. In recent years, the world's advanced apple production countries have basically completed t...

AI Technical Summary

Problems solved by technology

However, in the research on apple tissue culture, the main problem at present is: in the process of mass production, with the increase of the number of subcultures, the tissue culture seedlings are prone to vitrification, seedling height reduction, and growth vigor decline, which in turn affects proliferation and rooting culture. , resulting in low production efficiency and high production costs

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