High efficient JM bacterium technology for processing high salt heavy metal degradation-resistant organic wastewater, killing bacteria and removing odor

Abstract

The invention belongs to the technical field of microbiological wastewater processing, and specifically relates to a high efficient JM bacterium technology for processing high salt heavy metal degradation-resistant organic wastewater, killing bacteria and removing odor. The JM bacterium is a microbial composite inoculant, which comprises Haitobacter sufflavus Tih-w37, acinetobacter radioresistens,bacillus subtilis, bacillus licheniformis, brewer's yeast, trichoderma sp, ochrobactrum, cryytococcus neoformans, and bacillus cereus. The provided microbial composite inoculant has a stable structure and comprehensive functions, and does not have any side effect. The microbial flora can produce enzymes efficiently and proliferates quickly. Compared with EM bacteria, the JM bacteria are more efficient, more convenient, quicker, and more economic, and do have any toxicity or salt.

Description

technical field

[0001] The invention belongs to the technical field of microbial treatment of wastewater, in particular to a high-efficiency JM bacteria technology for the treatment of high-salt heavy metal refractory organic wastewater and antibacterial and deodorizing. Background technique

[0002] The treatment of high-salt heavy metals and deep concentrations of refractory organic wastewater has always been a difficult point in this industry. The water quality characteristics of high-salt and high-concentration organic wastewater: First, the concentration of organic matter is high, and COD is generally above 2000mg / L, and some even reach tens of thousands or even hundreds of thousands of mg / L. Relatively speaking, BOD is low, and many wastewater BOD and COD The ratio is less than 0.3; second, the composition is complex, and the organic matter in wastewater containing toxic substances is mostly aromatic compounds and heterocyclic compounds, and also contains sulfides, nit...

Examples

Embodiment 1

[0062] The preparation of embodiment 1 microbial composite bacterial agent

[0063] (1) Fermentation of various bacteria

[0064] Medium: LB;

[0065] Culture conditions: Shake the flask at 37°C until the bacterial concentration is about 10 8 cfu / ml. Usually 2x10 8 ~4x10 8 cfu / ml.

[0066] The slopes of Haitobacter sufflavus Tih-w37, Acinetobacter radioresistant, Bacillus subtilis, Bacillus licheniformis, Saccharomyces cerevisiae, Bacillus pallidum, Trichozyme, Geotrichum candidum, Bacillus cereus and Mucor The species were inoculated into LB liquid medium, and each strain was cultured separately.

[0067] Haitobacter sufflavus Tih-w37 is a microorganism with independent intellectual property rights, which is preserved in the China Center for Type Culture Collection (CCTCC) by Hao Wei, the inventor of the application, and selected into the National Natural Science and Technology Resource Platform as a new genus.

[0068] Haitobacter sufflavus Tih-w37 was deposited in Ch...

Embodiment 2

[0077] Embodiment 2 chicken manure waste water continuous treatment

[0078] Firstly, sterilize the very disgusting and smelly chicken manure wastewater and treat insect eggs. The specific method is as follows:

[0079] According to one thousandth of the total mass of waste water, dimethyl chloride quaternary ammonium salt bactericide (mass fraction is 3%) is added in the chicken manure waste water with very disgusting smell. Then in a special 2m with agitator 3 In the waste pit, add the chicken manure waste water (solid content rate is about 5%) of 1000kg, according to the 6 ‰ of chicken manure waste water quality, add the microbial composite bacterium I 6kg that obtains in the embodiment 1, described microbial composite bacterium I The effective bacteria content is greater than 2×10 8 cfu / ml, at 30 degrees, continuous aerated fermentation for 8 hours, the pH value is about 6, and the effective bacteria content is greater than 0.5×10 8 cfu / ml, about 5% solid content, obtai...

Embodiment 3

[0088] Embodiment 3 Continuous treatment of landfill leachate

[0089] First, weigh 200kg of landfill landfill leachate with a disgusting smell, and first settle to remove the precipitated sludge. Next, take 20 kilograms and put it in a 50 kilograms round plastic barrel with a diameter of 38.5cm and a height of 58cm, pass through the aeration pump for aeration, and add the 5‰ of the landfill leachate quality obtained in Example 1 Microbial compound bacterial agent I 0.1kg, its effective bacterial content is greater than 2×10 8 cfu / ml, at 30°C, continuous aeration treatment for 8 hours, the odor disappeared, and a treatment solution with a pH value of about 6 and containing a large amount of microorganisms was obtained.

[0090] Then, add new disgusting smell landfill leachate every two hours according to 30% of the initial landfill total mass of the treatment liquid containing a large number of microorganisms, and then the disgusting smell disappears quickly, that is, first a...

technical field

[0001] The invention belongs to the technical field of microbial treatment of wastewater, in particular to a high-efficiency JM bacteria technology for the treatment of high-salt heavy metal refractory organic wastewater and antibacterial and deodorizing. Background technique

[0002] The treatment of high-salt heavy metals and deep concentrations of refractory organic wastewater has always been a difficult point in this industry. The water quality characteristics of high-salt and high-concentration organic wastewater: First, the concentration of organic matter is high, and COD is generally above 2000mg / L, and some even reach tens of thousands or even hundreds of thousands of mg / L. Relatively speaking, BOD is low, and many wastewater BOD and COD The ratio is less than 0.3; second, the composition is complex, and the organic matter in wastewater containing toxic substances is mostly aromatic compounds and heterocyclic compounds, and also contains sulfides, nit...

Examples

Embodiment 1

[0062] The preparation of embodiment 1 microbial composite bacterial agent

[0063] (1) Fermentation of various bacteria

[0064] Medium: LB;

[0065] Culture conditions: Shake the flask at 37°C until the bacterial concentration is about 10 8 cfu / ml. Usually 2x10 8 ~4x10 8 cfu / ml.

[0066] The slopes of Haitobacter sufflavus Tih-w37, Acinetobacter radioresistant, Bacillus subtilis, Bacillus licheniformis, Saccharomyces cerevisiae, Bacillus pallidum, Trichozyme, Geotrichum candidum, Bacillus cereus and Mucor The species were inoculated into LB liquid medium, and each strain was cultured separately.

[0067] Haitobacter sufflavus Tih-w37 is a microorganism with independent intellectual property rights, which is preserved in the China Center for Type Culture Collection (CCTCC) by Hao Wei, the inventor of the application, and selected into the National Natural Science and Technology Resource Platform as a new genus.

[0068] Haitobacter sufflavus Tih-w37 was deposited in Ch...

Embodiment 2

[0077] Embodiment 2 chicken manure waste water continuous treatment

[0078] Firstly, sterilize the very disgusting and smelly chicken manure wastewater and treat insect eggs. The specific method is as follows:

[0079] According to one thousandth of the total mass of waste water, dimethyl chloride quaternary ammonium salt bactericide (mass fraction is 3%) is added in the chicken manure waste water with very disgusting smell. Then in a special 2m with agitator 3 In the waste pit, add the chicken manure waste water (solid content rate is about 5%) of 1000kg, according to the 6 ‰ of chicken manure waste water quality, add the microbial composite bacterium I 6kg that obtains in the embodiment 1, described microbial composite bacterium I The effective bacteria content is greater than 2×10 8 cfu / ml, at 30 degrees, continuous aerated fermentation for 8 hours, the pH value is about 6, and the effective bacteria content is greater than 0.5×10 8 cfu / ml, about 5% solid content, obtai...

Embodiment 3

[0088] Embodiment 3 Continuous treatment of landfill leachate

[0089] First, weigh 200kg of landfill landfill leachate with a disgusting smell, and first settle to remove the precipitated sludge. Next, take 20 kilograms and put it in a 50 kilograms round plastic barrel with a diameter of 38.5cm and a height of 58cm, pass through the aeration pump for aeration, and add the 5‰ of the landfill leachate quality obtained in Example 1 Microbial compound bacterial agent I 0.1kg, its effective bacterial content is greater than 2×10 8 cfu / ml, at 30°C, continuous aeration treatment for 8 hours, the odor disappeared, and a treatment solution with a pH value of about 6 and containing a large amount of microorganisms was obtained.

[0090] Then, add new disgusting smell landfill leachate every two hours according to 30% of the initial landfill total mass of the treatment liquid containing a large number of microorganisms, and then the disgusting smell disappears quickly, that is, first a...

Images