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Efficient extremely-trace gene typing technology based on Wafergen Smartchip system

A genotyping and micro-quantity technology, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems of high single detection cost and low throughput, and achieve the improvement of detection automation level and detection throughput. The effect of reducing the amount of PCR reagents

Inactive Publication Date: 2018-08-17
安徽微分基因科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the SNP detection technologies using probes or PCR methods on the market are generally probe capture, QPCR, etc. These technologies have problems of high single detection cost or low throughput. The Wafergen smartchip platform can solve these problems very well. It has the advantages of low cost, high throughput and high accuracy

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A high-efficiency micro-volume genotyping technology based on the Wafergen Smartchip system, which specifically includes the following steps:

[0018] Step 1, debug the MSND (WaferGen Biosystems SmartChip MultisampleNanodispenser) dispensing equipment, and use the MSND (WaferGen Biosystems SmartChip MultisampleNanodispenser) own software to set the dispensing program;

[0019] Step 2, configure the reaction enzyme reagent according to the needs, and then add the reaction enzyme reagent to the 384-well plate, and add 5 microliters of the enzyme reagent to each well;

[0020] Step 3: Add the DNA samples to the wells of the 384-well plate that have been added with enzyme reagents, 5 microliters per well;

[0021] Step 4, place the chip with 5184 nanowells into MSND (WaferGen Biosystems SmartChip Multisample Nanodispenser), place the 384-well plate equipped with samples and enzyme reagents on top of MSND (WaferGen Biosystems SmartChip Multisample Nanodispenser), and then pe...

Embodiment 2

[0026] A high-efficiency micro-volume genotyping technology based on the Wafergen Smartchip system, which specifically includes the following steps:

[0027] Step 1, debug the MSND (WaferGen Biosystems SmartChip MultisampleNanodispenser) dispensing equipment, and use the MSND (WaferGen Biosystems SmartChip MultisampleNanodispenser) own software to set the dispensing program;

[0028] Step 2, configure the reaction enzyme reagent according to the needs, and then add the reaction enzyme reagent to the 384-well plate, and add 8.4 microliters of enzyme reagent to each well;

[0029] Step 3, add the DNA samples to the wells of the 384-well plate that have been added with enzyme reagents, 5.6 microliters per well;

[0030] Step 4, place the chip with 5184 nanowells into MSND (WaferGen Biosystems SmartChip Multisample Nanodispenser), place the 384-well plate equipped with samples and enzyme reagents on top of MSND (WaferGen Biosystems SmartChip Multisample Nanodispenser), and then pe...

Embodiment 3

[0035] A high-efficiency micro-volume genotyping technology based on the Wafergen Smartchip system, which specifically includes the following steps:

[0036] Step 1, debug the MSND (WaferGen Biosystems SmartChip MultisampleNanodispenser) dispensing equipment, and use the MSND (WaferGen Biosystems SmartChip MultisampleNanodispenser) own software to set the dispensing program;

[0037] Step 2, configure the reaction enzyme reagent according to the needs, and then add the reaction enzyme reagent to the 384-well plate, and add 10 microliters of enzyme reagent to each well;

[0038] Step 3, add the DNA samples to the wells of the 384-well plate that have been added with enzyme reagents, 8 microliters per well;

[0039]Step 4, place the chip with 5184 nanowells into MSND (WaferGen Biosystems SmartChip Multisample Nanodispenser), place the 384-well plate equipped with samples and enzyme reagents on top of MSND (WaferGen Biosystems SmartChip Multisample Nanodispenser), and then perfor...

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PUM

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Abstract

The invention discloses an efficient extremely-trace gene typing technology based on a Wafergen Smartchip system. The technology specifically comprises the following steps: S1, debugging an MSND (WaferGen Biosystesms SmartChip Multisample Nanodispenser) liquid distributing device, and setting a liquid distributing program by utilizing the self software of the MSND (WaferGen Biosystesms SmartChip Multisample Nanodispenser); S2, preparing a reaction enzyme reagent according to the demands, and adding the reaction enzyme reagent into a 384 pore plate, wherein 5-10 microlitres of the enzyme reagent is added into each pore position; and S3, adding the DNA sample into pores added with the enzyme reagent in the 384 pore plate, wherein each pore position contains 5-8 microlitres of the DNA sample.The chip with 5184 nanopores is adopted, each nanopore can carry out the 100nl-level PCR reaction, one chip can perform 5184 SNP typing detection at one time, compared with the other SNP typing technologies, the technology provided by the invention has the advantages that the reagent needed by detection can be greatly reduced, the automation level of the detection can be promoted, meanwhile, by taking the Smartchip as the basis, the use amount of the PCR reagent in the SNP gene typing is reduced, and thus the detection throughput is effectively promoted.

Description

technical field [0001] The invention relates to the technical field of genotyping, in particular to a high-efficiency micro-quantity genotyping technology based on the Wafergen Smartchip system. Background technique [0002] SNP (single nucleotide polymorphism, SNP) refers to the DNA sequence diversity caused by a single nucleotide difference on the genome. There are many SNP sites in human genes, and SNP differences directly lead to differences in phenotypes of different people, which further reflect differences in sensitivity to pathogenic factors in the environment. Therefore, the use of SNP detection technology can predict the risk of individual diseases. At present, the SNP detection technologies using probes or PCR methods on the market are generally probe capture, QPCR, etc. These technologies have problems of high single detection cost or low throughput. The Wafergen smartchip platform can solve these problems very well. It has the advantages of low cost, high throu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6858C12Q1/6837
CPCC12Q1/6837C12Q1/6858
Inventor 傅延张鸿张慧徐子静
Owner 安徽微分基因科技有限公司