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Detection method of isomaltose, isomaltotriose, maltose and panose in rice wine

A technology of isomalt and isomalt, applied in the field of chemical analysis

Active Publication Date: 2021-02-09
INTEGRATED TECH SERVICES CENT OF SHAOXINGENTRY EXIT INSPECTION & QUARANTINE BUREAU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The use of ion chromatography-mass spectrometry for the determination of four oligosaccharides in food, especially isomaltose, isomaltotriose, maltose, and panose, has not been reported yet.

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  • Detection method of isomaltose, isomaltotriose, maltose and panose in rice wine
  • Detection method of isomaltose, isomaltotriose, maltose and panose in rice wine
  • Detection method of isomaltose, isomaltotriose, maltose and panose in rice wine

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Embodiment Construction

[0043] The present invention is specifically described below by the embodiment, only for further illustrating the present invention, can not be interpreted as the limitation of protection scope of the present invention, the technical engineer of this field can make some non-essential improvements and improvements to the present invention according to the content of the above-mentioned invention Adjustment.

[0044] according to Figure 1 to Figure 9 The reference figure of the experimental data, where Figure 5 to Figure 9 Among them, (1), (2), (3) and (4) refer to the positions and parameters of the four oligosaccharides in the chart, namely, isomaltose, isomaltotriose, maltose and panose, respectively. This embodiment discloses A method for detecting isomaltose, isomaltotriose, maltose and panose in rice wine, comprising the following steps:

[0045] (1) Preparation of samples to be tested: each take 2.0g Yuan red wine (dry rice wine), 1.0g Jiafan wine (semi-dry rice wine)...

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Abstract

The invention discloses a method for detecting isomaltose, isomaltotriose, maltose and panose in rice wine. Diluted wine sample with C 18 The solid phase extraction column is purified, and the purified sample solution is injected into the ion chromatography system, using CarboPac TM PA10 sugar analysis special column chromatographic column separation, NaOH solution elution, the effluent after the column is injected into the tandem quadrupole mass spectrometry system, and the multiple reaction monitoring mode is used to detect the isomaltose, isomaltotriose, maltose, pan Sugar, qualitatively confirmed by retention time and ion ratio, and quantified by standard solution external standard method. The method has the characteristics of simple and rapid sample pretreatment, good purification effect, high sensitivity, and simultaneous quantitative and qualitative confirmation.

Description

technical field [0001] The invention belongs to the field of chemical analysis, relates to ion chromatography-tandem mass spectrometry (IC-MS / MS), specifically refers to the ions of four kinds of oligosaccharides in rice wine, isomaltose, isomaltotriose, maltose and panose Chromatography-tandem mass spectrometry detection method. Background technique [0002] Yellow rice wine, also known as old wine, is a famous national specialty in my country. It is one of the three major brewed wines (rice wine, wine and beer) in the world. Yellow rice wine contains functional amino acids, peptides, sugars (mainly oligosaccharides), and inorganic elements. , polyphenols and other biologically active ingredients that are beneficial to the human body. These functional components endow yellow rice wine with unique health care functions. Sugar is one of the main components in rice wine, mainly derived from residual sugar and dextrin that have not been fully fermented during the production pro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/14
CPCG01N30/02G01N30/14
Inventor 胡贝贞韩超沈燕董文洪宋伟华鲁小红
Owner INTEGRATED TECH SERVICES CENT OF SHAOXINGENTRY EXIT INSPECTION & QUARANTINE BUREAU