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Baixi pig LYRM2 gene CDS region cloning method

A cloning method, a technique for white-washing pigs, applied in the biological field, can solve the problems of LYRM2 gene expression and function that have not been reported in the literature.

Inactive Publication Date: 2018-08-24
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The gene is located in the 10.2 region of human chromosome 1, with a full length of 315321322bp, including 3 introns, encoding 88 amino acids, but there is no literature report on the expression and function of the LYRM2 gene in pig tissues

Method used

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  • Baixi pig LYRM2 gene CDS region cloning method
  • Baixi pig LYRM2 gene CDS region cloning method
  • Baixi pig LYRM2 gene CDS region cloning method

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Embodiment Construction

[0013] Embodiment of the present invention: Cloning method of CDS region of LYRM2 gene of white-washed pig

[0014] Materials and methods

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Abstract

The invention discloses a Baixi pig LYRM2 gene CDS region cloning method. Through the successful building of a Baixi pig LYRM2 gene CDS region, the technical support is provided for the study on the expression and distribution of LYRM2 protein in pig biological species; the method basis is provided for the study of biological influence of the LYRM2 protein on the pig; the theoretical basis is provided for the breeding and culture of the Baixi pig in the molecule aspect; the complicated steps during the study of obtaining the Baixi pig LYRM2 gene CDS region are avoided.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a method for cloning the CDS region of the LYRM2 gene of white-washed pigs Background technique [0002] LYRM2 gene is a highly expressed gene screened from CD133+ cells with liver metastases from intestinal cancer through genome-wide expression profile chips. CD133+ is the basis of stem cell surface marker gene research and is widely used in solid tumor research. LYRM2 gene is highly expressed in CD133+ cells Whether it is related to cancer needs further research. The LYRM2 gene belongs to the LYR family, and LYRM proteins have been identified as auxiliary subunits or assembly factors of the mitochondrial OXPHOS complex, and have been shown to interact with ancient mitochondrial proteins and were identified in mouse mitochondria. The gene is located in the 10.2 region of human chromosome 1, with a full length of 315321322bp, including 3 introns, encoding 88 amino acids, but there i...

Claims

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Application Information

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IPC IPC(8): C12N15/10
CPCC12N15/1096
Inventor 陈祥苑红霞敖叶陈伟
Owner GUIZHOU UNIV