Gene capable of enhancing anti-inflammatory capability of human adipose-derived stem cells and application of gene

A technology of mesenchymal stem cells and adipose tissue, which is applied in the gene and its application field to enhance the anti-inflammatory ability of human adipose-derived mesenchymal stem cells. It can solve the problems of stem cell injection dosage and treatment time to be explored, so as to delay the progress of renal failure and optimize the method , enhance the effect of anti-inflammatory ability

Inactive Publication Date: 2018-08-24
THE AFFILIATED HOSPITAL OF XUZHOU MEDICAL UNIV
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Problems solved by technology

At present, the research is still in the stage of animal experiments, and t

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Embodiment Construction

[0010] The technical solution of the present invention will be further described below in conjunction with the examples.

[0011] 1. When the lentivirus is used as the carrier for GNDF gene overexpression transfection, the virus calibration and transfection concentration should be reasonably controlled and adjusted. 2. THP-1-derived macrophages were induced by appropriate concentrations of PMA, LPS, and INF-γ to simulate the inflammatory microenvironment in vivo. When macrophages and stem cells are co-cultured, the ratio of cells in the upper and lower chambers of the transwell needs to be constantly explored. 3. When injecting GDNF-AMSCs into UUO mouse renal fibrosis model for treatment, the dose of stem cell injection and treatment time need to be regulated.

[0012] (1) Stem cells are cultured in a certain scale in vitro (2) Gene transfection and modification of stem cells with lentiviral vector (the construction of GDNF lentiviral vector was completed by Shanghai Jikai Ge...

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Abstract

The invention discloses a gene capable of enhancing the anti-inflammatory capability of human adipose-derived stem cells (AMSCs) and application of the gene. A nucleotide sequence of the gene is shownas SEQ ID NO: 1. The invention further provides the application of the gene capable of enhancing the anti-inflammatory capability of the human adipose-derived stem cells to a preparation process of amedicine for treating kidney diseases. The gene is a glial cell line-derived neurotrophic factor GDNF. The GDNF modified AMSCs can be used for optimizing the effects of treating kidney fibrosis and delaying a renal failure progress of pure adipose-derived stem cells. According to the gene disclosed by the invention, related researches of the kidney diseases are carried out by applying the GDNF modified AMSCs for the first time, a stem cell therapy method is optimized and a novel thought of traditional cell therapy is explored.

Description

technical field [0001] The invention belongs to the technical field of stem cells and kidney diseases, and relates to a gene for enhancing the anti-inflammatory ability of human adipose-derived mesenchymal stem cells and its application. Background technique [0002] Renal interstitial fibrosis is the necessary pathway for the progression of renal diseases caused by various reasons, and it is also a common pathological state of end-stage renal disease (ESRD). Renal failure is a pathological state in which various chronic kidney diseases develop to a later stage and cause partial or complete loss of renal function. Kidney failure can be divided into acute renal failure and chronic renal failure. Acute renal failure progresses rapidly, usually due to insufficient blood supply to the kidneys (such as trauma or burns), impaired function of the kidneys due to blockage by some factors, or exposure to poisons injury, causing acute renal failure. The main reason for chronic renal ...

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Application Information

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IPC IPC(8): C12N15/12A61K48/00A61P13/12
CPCA61K48/005A61P13/12C07K14/4756
Inventor 孙东
Owner THE AFFILIATED HOSPITAL OF XUZHOU MEDICAL UNIV
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