Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Gene capable of enhancing anti-inflammatory capability of human adipose-derived stem cells and application of gene

A technology of mesenchymal stem cells and adipose tissue, which is applied in the gene and its application field to enhance the anti-inflammatory ability of human adipose-derived mesenchymal stem cells. It can solve the problems of stem cell injection dosage and treatment time to be explored, so as to delay the progress of renal failure and optimize the method , enhance the effect of anti-inflammatory ability

Inactive Publication Date: 2018-08-24
THE AFFILIATED HOSPITAL OF XUZHOU MEDICAL UNIV
View PDF2 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research is still in the stage of animal experiments, and the injection dose and treatment time of stem cells are still to be explored

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0010] The technical solution of the present invention will be further described below in conjunction with the examples.

[0011] 1. When the lentivirus is used as the carrier for GNDF gene overexpression transfection, the virus calibration and transfection concentration should be reasonably controlled and adjusted. 2. THP-1-derived macrophages were induced by appropriate concentrations of PMA, LPS, and INF-γ to simulate the inflammatory microenvironment in vivo. When macrophages and stem cells are co-cultured, the ratio of cells in the upper and lower chambers of the transwell needs to be constantly explored. 3. When injecting GDNF-AMSCs into UUO mouse renal fibrosis model for treatment, the dose of stem cell injection and treatment time need to be regulated.

[0012] (1) Stem cells are cultured in a certain scale in vitro (2) Gene transfection and modification of stem cells with lentiviral vector (the construction of GDNF lentiviral vector was completed by Shanghai Jikai Ge...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a gene capable of enhancing the anti-inflammatory capability of human adipose-derived stem cells (AMSCs) and application of the gene. A nucleotide sequence of the gene is shownas SEQ ID NO: 1. The invention further provides the application of the gene capable of enhancing the anti-inflammatory capability of the human adipose-derived stem cells to a preparation process of amedicine for treating kidney diseases. The gene is a glial cell line-derived neurotrophic factor GDNF. The GDNF modified AMSCs can be used for optimizing the effects of treating kidney fibrosis and delaying a renal failure progress of pure adipose-derived stem cells. According to the gene disclosed by the invention, related researches of the kidney diseases are carried out by applying the GDNF modified AMSCs for the first time, a stem cell therapy method is optimized and a novel thought of traditional cell therapy is explored.

Description

technical field [0001] The invention belongs to the technical field of stem cells and kidney diseases, and relates to a gene for enhancing the anti-inflammatory ability of human adipose-derived mesenchymal stem cells and its application. Background technique [0002] Renal interstitial fibrosis is the necessary pathway for the progression of renal diseases caused by various reasons, and it is also a common pathological state of end-stage renal disease (ESRD). Renal failure is a pathological state in which various chronic kidney diseases develop to a later stage and cause partial or complete loss of renal function. Kidney failure can be divided into acute renal failure and chronic renal failure. Acute renal failure progresses rapidly, usually due to insufficient blood supply to the kidneys (such as trauma or burns), impaired function of the kidneys due to blockage by some factors, or exposure to poisons injury, causing acute renal failure. The main reason for chronic renal ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/12A61K48/00A61P13/12
CPCA61K48/005A61P13/12C07K14/4756
Inventor 孙东
Owner THE AFFILIATED HOSPITAL OF XUZHOU MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com