Process for production and extraction of dihydrolipoic acid

A technology of dihydrolipoic acid and microbial culture, applied in the direction of fat oil/fat production, fat production, bacteria used in food preparation, etc., can solve problems such as difficult to separate and stabilize DHLA

Active Publication Date: 2018-08-28
PREMIER RES LABS
View PDF5 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Apparently, it is difficult to isol

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Process for production and extraction of dihydrolipoic acid
  • Process for production and extraction of dihydrolipoic acid
  • Process for production and extraction of dihydrolipoic acid

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0083] DHLA batch fermentation method (batch mode)

[0084] (1) A 48-hour inoculum culture was prepared from stock cultures of Lactobacillus casei and Lactobacillus fermentum as follows.

[0085] (1a) Lactobacillus casei inoculum preparation:

[0086] Forty-eight hours prior to the mixing start date, the L. casei inoculum was prepared. Ten (10) grams of stock (0022) freeze-dried 10 billion CFU / g Lactobacillus casei (from Nebraska Cultures, Inc.) was added to Edge Biologicals in Memphis, TN. Co., Ltd. Catalog No. B-0965 (Edge Biologicals, Inc. Catalog No. B-0965 Memphis, TN) provided 500mL MRS fermentation broth. The inoculum was incubated at 37°C in an atmosphere of 90% N2:5% CO2:5% O2. After 48 hours of incubation, 500 mL of the inoculum was washed twice with phosphate buffered saline using a centrifuge. The washed pellet was then reconstituted with a total of 100 mL of phosphate buffer and combined into one vessel to inoculate the DHLA fermentation batch.

[0087] (1b) ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

A process for production and extraction of dihydrolipoic acid (DHLA) relates to the biotransformation of R-lipoic acid to dihydrolipoic acid. The process ferments the lipoic acid with bacteria selected from the genus of any of the following bacteria: Lactobacillus, Enterococcus, Pediococcus, or Bacillus. More particularly the present invention relates to a method for the extraction of DHLA utilizing a non-traditional solvent system, namely a food oil. Generally the DHLA that is produced in the present method is isolated from cells which have been inactivated or killed.

Description

technical field [0001] The present invention relates to the biotransformation of R-lipoic acid to dihydrolipoic acid (DHLA). The method ferments lipoic acid with bacteria selected from any of the following bacterial genera: Lactobacillus, Enterococcus, Pediococcus or Bacillus. More specifically, the present invention relates to methods for extracting DHLA using non-traditional solvent systems (ie, food oils). Typically, DHLA produced in the methods of the invention is isolated from cells that have been inactivated or killed. Background technique [0002] Dihydrolipoic acid is a valuable nutrient. Its production and isolation are made difficult by its ease of oxidation to its disulfide form (ie, lipoic acid) under a variety of standard conditions. [0003] DHLA is typically produced in vivo during normal metabolic activity through the redox conversion of lipoic acid or alpha-lipoic acid (ALA). However, the body usually only produces enough DHLA to aid in metabolic functio...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12P11/00C12P7/00C12P7/40
CPCC11B3/003C11B1/025A23L33/115C12P11/00C11B3/006A23V2400/125A23L29/04
Inventor 罗伯特·J·马歇尔尼克拉斯·拉宾斯克林德赛·邓纳姆迈克尔·拉特科维奇沃尔特·特里克斯科
Owner PREMIER RES LABS
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap