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Methods of generating microparticles and porous hydrogels using microfluidics

A technology of microfluidic devices and microparticles, which can be used in inert gas generation, chemical instruments and methods, microcapsules, etc., and can solve problems such as limited practicability and processing conditions.

Active Publication Date: 2018-08-31
UNIVERSITY OF WYOMING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

ICCs have been developed for a variety of scientific and technological applications, but their utility is still limited by the harsh processing conditions required to dissolve the particles to form the pore framework

Method used

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  • Methods of generating microparticles and porous hydrogels using microfluidics
  • Methods of generating microparticles and porous hydrogels using microfluidics
  • Methods of generating microparticles and porous hydrogels using microfluidics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0081] Example 1: Formation of monodisperse polyethylene glycol diacrylate hydrogel microspheres by oxygen-controlled photopolymerization in a microfluidic device

[0082] Introduction

[0083] Acrylate-based polymers have attracted much attention due to their transparency, color change, robust mechanical properties, and elasticity (Ref. 1.1). Acrylates are readily photopolymerizable on an industrial scale and are widely used in chemical applications as adhesives, sealant composites, and protective coatings (Refs 1.1, 1.2). Acrylates are generally preferred over other synthetic monomers due to their biocompatibility and chemical versatility, allowing modification with a range of monofunctional or multifunctional groups (refs 1.3-1.4). Oligomeric acrylates can be used to produce highly crosslinked networks by photopolymerization methods (ref. 1.5). Based on these features, a class of photopolymerizable polyethylene glycol (PEG)-based hydrogels has been developed around acryla...

Embodiment 2

[0126] Embodiment 2: the method for reducing the oxygen concentration in nitrogen microjacket microfluidic device

[0127] Introduction

[0128] In this example, a nitrogen microjacketed microfluidic device was modified by adding a pre-purification channel for a Novec 7500 to further reduce the oxygen concentration in the device to lower levels. When the nitrogen pressure was increased, less photoinitiator (LAP) was required for the polymerization and correspondingly higher post-encapsulation viability of the cells was obtained. This new method can be used to produce size-controllable microgels that are compatible with commercially available flow cytometry methods that provide high levels of cell viability after encapsulation.

[0129] Materials and methods

[0130] cell culture

[0131] Human lung adenocarcinoma epithelial cells (A549) were used in this study and maintained at 10% fetal bovine serum (Life Technologies, USA), 1% Pen / Strep and 0.2% amphotericin B (Sigma-Aldr...

Embodiment 3

[0160] Example 3: Modeling hydrogel inverse opal structures using photodegradable microparticles

[0161] Inverse colloidal crystals (ICCs) are a product of the lost-wax manufacturing process in which colloidal particles assemble into an ordered matrix in the presence of a liquid continuous phase. The particles are removed after solidification of the continuous phase, leaving behind a structured pore network. ICCs have been developed for various scientific and technological applications, but their practicality is still limited by the harsh processing conditions required to dissolve the particles to form the pore framework.

[0162] In this example, we show a new method for the production of ICCs based on a method involving the synthesis of photodegradable polyethylene glycol diacrylate particles. Since the degradation of the particle phase requires only optical illumination, particle assemblies can be eroded within tightly confined microchannels, creating a microfluidic-integ...

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Abstract

Provided herein are methods utilizing microfluidics for the oxygen-controlled generation of microparticles and hydrogels having controlled microparticle sizes and size distributions and products fromprovided methods. The included methods provide the generation of microparticles by polymerizing an aqueous solution dispersed in a non-aqueous continuous phase in an oxygen-controlled environment. Theprocess allows for control of size of the size of the aqueous droplets and, thus, control of the size of the generated microparticles which may be used in biological applications.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of and priority to U.S. Provisional Patent Application No. 62 / 285,352, filed October 26, 2015, the entire contents of which are incorporated herein by reference. [0003] Statement Regarding Federally Sponsored Research or Development [0004] This invention was made with U.S. Government support under the National Science Foundation Faculty Early Career Development (CAREER) program (BBBE 1254608) and the Wyoming Biomedical Research Excellence Program IDeA Network through NIH grants to J. Oakey (P20RR016474 and P20GM103432) obtained. The US Government has certain rights in this invention. Background technique [0005] PEG-based hydrogels have been widely used as drug delivery and tissue scaffold materials. Common among the polymers that form PEG hydrogels is a photopolymerizable acrylate in the form of polyethylene glycol diacrylate (PEGDA). Recently, microfluidics and microfabricat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/50A61K9/58B01J2/02B01J13/14B01J19/14G03F7/039
CPCB01J19/0093B01J2219/00792B01J2219/00936C08F216/125C08F2/48B01J13/0052B01J2/06A61K9/5026C08F2/32C08F222/102B01F23/41B01F25/4331B01F33/3011C08J3/075A61L27/18A61L27/52A61L27/56A61L27/16A61L27/38A61K35/12C12N5/0012C08J9/26C08J2201/046C08J2205/022C08J2335/02C08J2345/00C08J2207/10
Inventor J·奥基K·克鲁特拉梅利斯B·夏
Owner UNIVERSITY OF WYOMING
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