Planting method of fritillaria cirrhosa
A planting method and fritillary technology, applied in the agricultural field, can solve the problems of difficult seedling production, resource exhaustion, and low seedling preservation rate.
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[0014] A planting method of Fritillaria cirrhosa, including the following steps:
[0015] Step 1. Take the bulbs of Fritillaria cirrhosa and inoculate them in hydroponic nutrient solution containing 300~500mg / L of colchicine and culture for one week;
[0016] Step 2. Wash the colchicine-treated Fritillaria cirrhosae and inoculate it in a nutrient solution containing no colchicine for one week. After the bulbs of Fritillaria cirrhosa grow thick polyploid adventitious roots, take the polyploidy Adventitious roots are inoculated into callus medium and cultured for 5-10 days to obtain polyploid callus; the formula of callus medium is callus medium and the formula is 1 / 2MS medium+NAA0.1- 0.2mg / L+IAA 0.2-0.3mg+6-BA0.1- 0.2mg / L+5-10g / L agar+30-35g / L sucrose+5-10g / L potato juice+2-3g / L apple Juice; step three, inoculate the callus into the differentiation tissue culture medium and culture for 5-10 days to obtain polyploid Fritillaria cirrhosa seedlings; the differentiation tissue culture ...
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