PpCER1-2 gene, vector and application of PpCER1-2 gene in improvement of drought resistance of gramineous plants

A gene and carrier technology, applied in the field of plant breeding, can solve problems such as hindering plant drought resistance, lack, and lack of grass cuticle, and achieve the effects of improving plant drought resistance, slowing water loss, and enhancing crop yield reduction.

Inactive Publication Date: 2018-09-14
SOUTHWEST UNIV
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Problems solved by technology

[0004] In summary, the problem in the prior art is that there is still a lack of effective gene resources for improving grass cuticle characteristics, especially the lack of waxy synthetic genes on grass non-model plants, thereby hindering the use of genetic engineering. The application of technology to improve the characteristics of cuticle and improve the drought resistance of plants

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  • PpCER1-2 gene, vector and application of PpCER1-2 gene in improvement of drought resistance of gramineous plants
  • PpCER1-2 gene, vector and application of PpCER1-2 gene in improvement of drought resistance of gramineous plants
  • PpCER1-2 gene, vector and application of PpCER1-2 gene in improvement of drought resistance of gramineous plants

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[0026] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0027] The application principle of the present invention will be described in detail below in conjunction with the accompanying drawings.

[0028] The sequence of the PpCER1-2 gene provided in the embodiment of the present invention is: SEQ ID NO:1.

[0029] Such as figure 1 As shown, the construction method of the PpCER1-2 gene provided by the embodiments of the present invention includes the following steps:

[0030] S101: According to the bluegrass leaf transcriptome data, design the upstream primer: ATGGCGACCAGGCCGGG (SEQ ID NO: 2), the downstream primer: TCAAGCTTTCGTCAGAGGGACGA (SEQ ID NO: 3), and use the first stra...

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Abstract

The invention belongs to the technical field of plant breeding and discloses a PpCER1-2 gene, a vector and application of the PpCER1-2 gene in improvement of the drought resistance of gramineous plants. The sequence of the PpCER1-2 gene is as shown in SEQ ID NO: 1. The application of the PpCER1-2 gene in improvement of the drought resistance of the gramineous plants is provided. Experiments provethat cuticle alkane substances of a PpCER1-2 gene transferred and ultra-expressed Brachypodium distachyon blade are increased, the water loss of the surface of the blade is relieved, the chlorophyll leaching rate is reduced, and the growth condition of seedlings in drought environment also proves that the PpCER1-2 gene can effectively improve the drought resistance of the gramineous plants. The gramineous plants are important grain crops and garden green land plants, and after the drought resistance of the gramineous plants is improved by the PpCER1-2 gene, the use amount of irrigation water can be reduced, meanwhile, the problem of crop yield reduction caused by water shortage in the high-temperature weather in summer can be solved, and the application prospect is good.

Description

technical field [0001] The invention belongs to the technical field of plant breeding, and in particular relates to a PpCER1-2 gene, a carrier and its application in improving the drought resistance of gramineous plants. Background technique [0002] During the growth and development of plants, they are often affected by many abiotic stresses such as drought, salinity, low temperature, etc.; drought stress is one of the most common stress factors in various environmental stresses; improving the drought resistance of plants has become a modern plant breeding It is one of the key problems that need to be solved urgently. During the long-term evolutionary process, higher plants gradually evolved a series of physiological or developmental mechanisms to respond to water stress in the environment. This adaptability of plants involves many fields such as plant morphology, physiology, biochemistry and molecular biology. The tolerance of higher plants to water stress has the same m...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/52C12N15/82A01H5/00A01H6/46
CPCC12N9/00C12N15/8273
Inventor 郭彦军倪郁王党军徐熠
Owner SOUTHWEST UNIV
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