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A method for regulating the plant structure of crops and a method for obtaining compact crops

A crop and plant type technology, applied in the field of plant genetic engineering, can solve the problems of long genetic breeding time, shorten the breeding time, time-consuming and laborious, etc., and achieve the effect of improving plant type structure and good practical application value

Active Publication Date: 2021-07-13
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Traditional genetic breeding is a relatively mature path, but genetic breeding takes a long time, is time-consuming and laborious, and may not be able to obtain the ideal plant type; but with the development of molecular biology, molecular biological means can greatly It is a better means to greatly shorten the breeding time and increase the probability of obtaining target plants
[0004] Plant structure is a major trait of crops. At present, in Rosaceae and Solanaceae, research on plant structure is not enough; especially in apples and tomatoes, the research needs to be further strengthened

Method used

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  • A method for regulating the plant structure of crops and a method for obtaining compact crops
  • A method for regulating the plant structure of crops and a method for obtaining compact crops
  • A method for regulating the plant structure of crops and a method for obtaining compact crops

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Experimental program
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Embodiment 1

[0042] This embodiment provides a method for regulating the plant structure of crops, that is, by regulating the expression of the crop SBP-Box gene to achieve the purpose of regulating the plant structure of crops; the base sequence of the SBP-Box gene is as shown in SEQ ID NO.1 Show.

[0043] The present embodiment provides a method for regulating and controlling the plant structure of crops, comprising the following steps:

[0044] 1.1 Select two suitable restriction sites, design the restriction sites on the primers through primer design, and introduce the restriction sites to both ends of the SBP-Box gene by PCR amplification or artificial synthesis;

[0045] 1.2 Digest the SBP-Box gene fragment with the restriction site and the pBI121 vector plasmid with double restriction enzymes, and recover by gel electrophoresis;

[0046] 1.3 Electrophoresis recovery to obtain SBP-Box fragment and pBI121 vector fragment;

[0047] 1.4 Ligate the SBP-Box fragment and the pBI121 vecto...

Embodiment 2

[0071] In this example, transcriptome analysis was performed on 3 transgenic lines (L4, L6 and L22) and 3 other non-transgenic lines (CK1, CK2 and CK3) obtained from transgenic tomato.

[0072] The 3 transgenic lines (L4, L6 and L22) and the other 3 and non-transgenic lines (CK1, CK2 and CK3) were respectively subjected to illumina transcriptome sequencing. The sequencing read length was 100bp, and each sample obtained more than 4Gclean data data. The obtained data are shown in Table 1.

[0073] Table 1 Statistical table of Clean data obtained by sequencing

[0074]

[0075] The distribution of reads on the genome is consistent with the distribution of genes on the chromosome, indicating that the sequencing results are unbiased.

[0076] Align the clean data of each sample with the reference gene and the reference genome by using the short reads alignment software SOAPaligner / SOAP2, allowing up to 5 base mismatches, and 87.30%-88.95% of the reads are aligned to the refere...

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Abstract

The invention provides a method for regulating the plant structure of crops, which belongs to the field of plant genetic engineering. The present invention adopts a method for regulating various agronomic traits of crops. The method realizes the regulation of crop plant type by regulating the expression of SBP-Box gene; and then obtains crops with compact plant type, suitable for dense planting, convenient harvesting, and lodging resistance. ; By transforming genes into crops, optimize the plant structure of crops, improve the lodging resistance of crops, increase the planting density of crops, and facilitate manual and mechanical harvesting; it has good practical application value.

Description

technical field [0001] The invention relates to the field of plant genetic engineering, in particular to a method for regulating the plant structure of crops and a method for obtaining crops with compact plant type. Background technique [0002] In agricultural production, the agronomic traits of crops have a great influence on the yield of crops. In agricultural research, the research on ideal plant type is carried out continuously. Researchers improve the yield of crops by constantly adjusting the plant type of crop plants. , cultivation, management, harvest performance. [0003] Traditional genetic breeding is a relatively mature path, but genetic breeding takes a long time, is time-consuming and laborious, and may not be able to obtain the ideal plant type; but with the development of molecular biology, molecular biological means can greatly It is a better means to greatly shorten the breeding time and increase the probability of obtaining target plants. [0004] Plant...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/82C12N15/29A01H5/00A01H6/82
CPCC07K14/415C12N15/8291
Inventor 张晓辉李锡香宋江萍邱杨王海平
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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