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Articular cavity liquid mesenchymal stem cell preparation for treating osteoarthritis and preparation method of preparation

An osteoarthritis and stem cell technology, applied in the field of biomedicine, can solve the problems of low immunogenicity and unstable treatment effect of mesenchymal stem cells, and achieve low immunogenicity, inhibition of immune rejection, and great differentiation potential. Effect

Pending Publication Date: 2018-09-18
THE SECOND PEOPLES HOSPITAL OF SHENZHEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Mesenchymal stem cells also have the characteristics of low immunogenicity, regulating the body's immune response, and strong ability to secrete cytokines, and have the potential to treat osteoarthritis; although mesenchymal stem cells have made great progress in the treatment of articular cartilage, mesenchymal stem cells have The therapeutic effect of mesenchymal stem cells is still unstable, and the curative effect needs to be further improved; therefore, we propose a joint cavity fluid mesenchymal stem cell preparation for the treatment of osteoarthritis and its preparation method

Method used

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Experimental program
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Effect test

Embodiment 1

[0020] A joint cavity fluid mesenchymal stem cell preparation for treating osteoarthritis, comprising the following components (by mass percentage): 20% of joint cavity fluid, 11% of mesenchymal stem cell culture medium, and 3.6% of human serum albumin , Electrolyte balance solution 2.5%, adenosine triphosphate 1.88%, sodium hyaluronate 1.24%, glucosamine 1.12%, sodium hyaluronate 0.79%, aceclofenac solution 0.55%, polyethylene glycol solution 2.45%, sodium alginate solution 1.86 %, carboxymethyl chitosan solution 2.55%, physiological saline as the balance.

[0021] Further, the electrolyte balance solution is Nalinger Acetate Injection.

[0022] A method for preparing a joint cavity fluid mesenchymal stem cell preparation for treating osteoarthritis, comprising the following steps:

[0023] Step 1: Prepare mesenchymal stem cell culture medium: prepare L-DMEM medium, add penicillin with a concentration of 90U / ml and streptomycin with a concentration of 90U / ml to the medium to...

Embodiment 2

[0030]A joint cavity fluid mesenchymal stem cell preparation for treating osteoarthritis, comprising the following components (by mass percentage): 23% of joint cavity fluid, 12% of mesenchymal stem cell culture fluid, and 3.8% of human serum albumin , Electrolyte balance solution 2.8%, adenosine triphosphate 1.94%, sodium hyaluronate 1.33%, glucosamine 1.24%, sodium hyaluronate 0.83%, aceclofenac solution 0.58%, polyethylene glycol solution 2.85%, sodium alginate solution 1.97 %, carboxymethyl chitosan solution 2.87%, physiological saline as the balance.

[0031] Further, the electrolyte balance solution is Nalinger Lactate Injection.

[0032] A method for preparing a joint cavity fluid mesenchymal stem cell preparation for treating osteoarthritis, comprising the following steps:

[0033] Step 1: Prepare mesenchymal stem cell culture medium: prepare L-DMEM medium, add penicillin with a concentration of 93U / ml and streptomycin with a concentration of 93U / ml to the medium to p...

Embodiment 3

[0040] A joint cavity fluid mesenchymal stem cell preparation for treating osteoarthritis, comprising the following components (by mass percentage): 27% of joint cavity fluid, 14% of mesenchymal stem cell culture fluid, and 4% of human serum albumin , Electrolyte balance solution 3.2%, adenosine triphosphate 2.11%, sodium hyaluronate 1.4%, glucosamine 1.32%, sodium hyaluronate 0.91%, aceclofenac solution 0.63%, polyethylene glycol solution 3.61%, sodium alginate solution 2.12 %, carboxymethyl chitosan solution 3.35%, physiological saline as the balance.

[0041] Further, the electrolyte balance solution is invert sugar electrolyte injection.

[0042] A method for preparing a joint cavity fluid mesenchymal stem cell preparation for treating osteoarthritis, comprising the following steps:

[0043] Step 1: Prepare mesenchymal stem cell culture medium: prepare L-DMEM medium, add penicillin with a concentration of 97U / ml and streptomycin with a concentration of 97U / ml to the mediu...

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Abstract

The invention discloses an articular cavity liquid mesenchymal stem cell preparation for treating osteoarthritis. The preparation contains the following components in percentage by weight: 20%-30% ofarticular cavity liquid, 11%-15% of a mesenchymal stem cell culture solution, 1.12%-1.38% of glucosamine, 0.79%-0.98% of sodium hyaluronate and 0.55%-0.65% of an aceclofenac solution. The invention further discloses a preparation method of the articular cavity liquid mesenchymal stem cell preparation for treating osteoarthritis. The preparation method comprises the steps of preparing a mesenchymalstem cell culture solution, preparing mesenchymal stem cell suspension, preparing mesenchymal stem cell populations and preparing the mesenchymal stem cell preparation. A formula of the preparation is relatively reasonable, the articular cavity liquid is cultured into mesenchymal stem cells in vitro for treating the osteoarthritis, and the mesenchymal stem cell preparation has the treatment characteristics aiming at osteoarthritis in vitro, and the treatment effect to osteoarthritis can be remarkably improved after the preparation is transplanted in vivo; and the materials in the preparationmethod are easily available, and the equipment requirement is low, so that the preparation method has very good promotional effects.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a joint cavity fluid mesenchymal stem cell preparation for treating osteoarthritis and a preparation method thereof. Background technique [0002] Osteoarthritis is a typical senile disease, and its pathogenesis is still not very clear. Factors such as increased intraosseous pressure, cytokines, matrix metalloproteinases, free radicals, and immune responses can all cause osteoarthritis. Existing treatments There are three main ways to treat osteoarthritis: physical therapy, drug therapy, and surgery. Physical therapy mainly refers to moderate exercise. Moderate intensity of exercise can enhance physical function, thereby slowing the progression of osteoarthritis. Physical therapy also includes ultrasonic therapy, electromagnetic therapy, laser therapy, electrical stimulation therapy, etc. using sound, light, and electrical equipment. These therapies can more or less reduce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61K47/42A61K47/36A61K47/26A61K47/18A61P19/08A61P19/02C12N5/0775
CPCA61K47/183A61K47/26A61K47/36A61K47/42A61P19/02A61P19/08A61K35/28C12N5/0668
Inventor 王大平徐晓李兴福段莉熊建义欧阳侃朱飞燕刘威黄江鸿邓志钦蒯声政
Owner THE SECOND PEOPLES HOSPITAL OF SHENZHEN
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