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Method for stimulating quick growth of centipedegrass by preprocessing stolons through low-temperature plasmas

A low-temperature plasma, stolon technology, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve problems such as no research reports on the growth effect of turfgrass, no research reports on vegetative materials, etc. Growth-promoting effect

Inactive Publication Date: 2018-09-21
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The objects of low-temperature plasma treatment are all seeds, but so far there are no research reports on the treatment of vegetative materials (such as stolons) by this technology, and there are no research reports on the growth effect of turfgrass (including Pseudomonas spp.

Method used

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  • Method for stimulating quick growth of centipedegrass by preprocessing stolons through low-temperature plasmas
  • Method for stimulating quick growth of centipedegrass by preprocessing stolons through low-temperature plasmas
  • Method for stimulating quick growth of centipedegrass by preprocessing stolons through low-temperature plasmas

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) Select a 3-4cm long stolon of Pseudomonas spp. containing two nodes and two leaves, and soak the stolon in clear water at 30-35°C for 18 hours;

[0018] (2) Treat stolons with dielectric barrier helium low-temperature plasma, the processing power is 150W, the processing time is 10s, the distance between the plates is 2cm, the pressure is 150Pa, and the frequency is 13.56kHz;

[0019] (3) placing the treated stolons in distilled water at 30°C for 18 hours;

[0020] (4) Plant the soaked stolons into flowerpots and place them under light at 25-35°C for cultivation.

[0021] The present invention can effectively promote the growth of the lower part and the upper part of the grass ( figure 2 ). Underground growth parameters such as underground dry weight, root-to-shoot ratio, total root length, root volume, root surface area and average root diameter increased by 12.20%, 8.26%, 18.51%, 30.80%, 33.19% and 7.87% compared with the control. Growth parameters of shoots su...

Embodiment 2

[0023] (1) Select a 3-4cm long stolon of Pseudomonas spp. containing two nodes and two leaves, and soak the stolon in clear water at 30-35°C for 18 hours;

[0024] (2) Treat stolons with dielectric barrier helium low-temperature plasma, the processing power is 300W, the processing time is 15s, the distance between the plates is 2cm, the pressure is 150Pa, and the frequency is 13.56kHz;

[0025] (3) Soak the treated stolons in clear water at 30°C for 20 hours;

[0026] (4) Plant the soaked stolons into flowerpots and place them under light at 25-35°C for cultivation.

[0027] The present invention effectively promotes the growth of the lower part and the upper part of the grass ( image 3 ). Underground growth parameters such as underground dry weight, root-to-shoot ratio, total root length, root volume, root surface area and average root diameter increased by 40.67%, 19.01%, 32.41%, 56.12%, 63.23% and 30.32% compared with the control, and the aboveground Growth parameters s...

Embodiment 3

[0029] (1) Select a 3-4cm long stolon of Pseudomonas spp. containing two nodes and two leaves, and soak the stolon in clear water at 30-35°C for 18 hours;

[0030] (2) Treat stolons with dielectric barrier helium low-temperature plasma, the processing power is 450W, the processing time is 20s, the distance between the plates is 2cm, the pressure is 50Pa, and the frequency is 13.56kHz;

[0031] (3) Soak the treated stolons in clear water at 30°C for 24 hours;

[0032] (4) Plant the soaked stolons into flowerpots and place them under light at 25-35°C for cultivation.

[0033] The present invention can effectively promote the growth of the lower part and the upper part of the grass ( Figure 4 ). Underground growth parameters such as underground dry weight, root-to-shoot ratio, total root length, root volume, root surface area and average root diameter increased by 15.40%, 8.26%, 24.35%, 61.60%, 43.53% and 20.41% compared with the control, and the aboveground Growth parameters...

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Abstract

The invention relates to a method for stimulating quick growth of centipedegrass by preprocessing stolons through low-temperature plasmas. The method includes the following steps that 1, the centipedegrass stolons are soaked in clean water at the temperature of 30-35 DEG C for 18 hours; 2, the dielectric barrier helium low-temperature plasmas are used for processing the stolons under the processing conditions that the processing intensity is 150-450 W, the processing time is 10-20 s, the polar plate spacing is 2 cm, the pressure intensity is 150 Pa, and the frequency is 13.56 kHz; 3, the processed stolons are soaked in clean water at the temperature of 30 DEG C for 18-24 hours; 4, the soaked stolons are planted in flowerpots to be cultivated. By processing the centipedegrass stolons with the low-temperature plasmas, the dry weight of underground parts, the root shoot ratio, the total root length, the root volume, the root specific surface area and the average root diameter of the centipedegrass stolons can be increased by 12-41%, 8-20%, 18-33%, 30-62%, 33-64%, 20-61%, 7-22% and 21-36% respectively. The new method is easy and convenient to operate, low in cost and free of pollution,takes effect quickly, can effectively promote the growth of the underground parts and overground parts of the centipedegrass stolons.

Description

technical field [0001] The invention relates to a low-temperature plasma pretreatment method for stolons to stimulate the rapid growth of Pseudomonas, belonging to the field of turfgrass cultivation. Background technique [0002] Eremochloa ophiuroides (Munro.) Hack. is a perennial plant of the genus Centipede Grass in the Poaceae family. It is one of the three warm-season turfgrasses in the world and is widely distributed in the Yangtze River Basin and the south of China. It has the advantages of beautiful leaf shape, long green period, resistance to barrenness, less pests and diseases, and low maintenance level, so it is known as "the best warm-season turfgrass in China". False thrift grass is widely used in the establishment of garden lawns, rest lawns and slope protection lawns, especially suitable for water and soil conservation and large-scale landscape construction. In addition, false thrift grass is also a good forage. [0003] The production of pseudo-thrift grass...

Claims

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Application Information

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IPC IPC(8): A01G7/06
CPCA01G7/06
Inventor 李玲刘建秀宗俊勤郭海林陈静波李建建李丹丹汪毅郭爱桂
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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