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Paeonia suffruticosa peel flavonoid extraction process and extracts thereof

An extraction process and an extract technology, which are applied to the extraction process of flavonoids from peony peel and the field of extracts thereof, can solve problems such as waste of peony peel resources, and achieve the effect of being easy to operate.

Inactive Publication Date: 2018-09-28
ZHENGZHOU NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the phenomenon of massive waste of peony peel resources and better develop and utilize peony peel resources, the purpose of this invention is to provide a flavonoid extraction process and extracts from peony peel

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A process for extracting flavonoids from peony peel, comprising the following steps:

[0021] (1) Place the peony peel in the shade indoors, dry it in a blast drying oven at 60°C after a period of time, and then use a cyclone mill to make powder, and pass through a 100-mesh sieve to obtain peel powder;

[0022] (2) Take 1 g of peel powder, preferably, extract with ethanol volume fraction of 50%, liquid-solid ratio of 25mL / g, ultrasonic extraction temperature of 80°C, and ultrasonic power of 700W;

[0023] (3) Filter the extract with quantitative filter paper, and the yield of flavonoids is (1.728±0.015)%.

[0024] The in vitro antioxidant activity of the extract of the above-mentioned a kind of peony peel flavonoids extraction process is as follows:

[0025] The ABTS scavenging ability measurement of the extract of a kind of peony peel flavonoid extraction process comprises the following steps: (1) weigh ABTS powder 0.096g, be settled to 25mL with distilled water, weig...

Embodiment 2

[0028] A process for extracting flavonoids from peony peel, comprising the following steps:

[0029] (1) Place the peony peel in the shade indoors, dry it in a blast drying oven at 60°C after a period of time, and then use a cyclone mill to make powder, and pass through a 100-mesh sieve to obtain peel powder;

[0030] (2) Take 1 g of peel powder, extract with ethanol volume fraction of 50-90%, liquid-solid ratio of 20mL / g, extraction temperature of 60°C, and ultrasonic power of 400W;

[0031] (3) Filter the extract with quantitative filter paper and measure the absorbance. Preferably, the maximum yield of flavonoids is 1.353% when the ethanol volume fraction is 60%.

Embodiment 3

[0033] A process for extracting flavonoids from peony peel, comprising the following steps:

[0034] (1) Place the peony peel in the shade indoors, dry it in a blast drying oven at 60°C after a period of time, and then use a cyclone mill to make powder, and pass through a 100-mesh sieve to obtain peel powder;

[0035] (2) Take 1 g of peel powder, extract with ethanol volume fraction of 70%, liquid-solid ratio of 5-30ml / g, extraction temperature of 60°C, and ultrasonic power of 400W;

[0036] (3) The extract is filtered with quantitative filter paper, and the absorbance is measured. The yield of flavonoids increases continuously with the increase of the liquid-to-solid ratio, but when the ratio of liquid to solid is greater than 20mL / g, the yield of flavones decreases gradually. Preferably, the flavones are the largest The yield reaches the maximum when the ratio of liquid to solid is 20mL / g.

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Abstract

The invention relates to a paeonia suffruticosa peel flavonoid extraction process and extracts thereof. The extraction process of paeonia suffruticosa 'Feng Dan' peel flavonoids is optimized with theyield of paeonia suffruticosa 'Feng Dan' peel flavonoids as the index, the influence of the four factors of ethanol volume fraction, liquid-material ratio, extraction temperature and ultrasonic poweron the yield of peel flavonoids is investigated, and the optimal extraction process parameters for paeonia suffruticosa 'Feng Dan' peel flavonoids are determined by analyzing the test result. An in-vitro anti-oxidation test on the flavonoid extracts in paeonia suffruticosa 'Feng Dan' peel shows that the flavonoid extracts in paeonia suffruticosa 'Feng Dan' peel have a scavenging effect on ABTS andDPPH within a concentration range selected in the test, the phenomenon of great waste of paeonia suffruticosa peel at present is avoided, and the paeonia suffruticosa peel resource is better developed and utilized.

Description

technical field [0001] The invention relates to a process for extracting flavonoids, in particular to a process for extracting flavonoids from peony peel and the extract thereof. Background technique [0002] Peony seed oil was recognized as a new resource food in 2011. 'Fengdan' peony (Paeonia suffruticosa'Feng Dan') is one of the varieties commonly used to extract peony seed oil. Health care functions such as reducing blood fat, lowering blood sugar, and sun protection are gradually being valued by people, and its planting area is expanding year by year, and its by-product-pericarp will also be produced in large quantities. [0003] Flavonoids are a class of biologically active substances with a variety of physiological activities, which can regulate the contraction and relaxation functions of the myocardium, and can be used to prevent cardiovascular diseases and other diseases; increase the toughness of blood vessels, reduce blood viscosity, and reduce cholesterol levels ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/71A61K36/65A61P9/12A61P9/10A61K131/00
CPCA61P9/10A61P9/12A61K36/65A61K36/71A61K2236/333
Inventor 陈刚马晓杨玉珍王国霞刘瑞霞
Owner ZHENGZHOU NORMAL UNIV
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