Multi-color super-resolution imaging system applied to 4pi microscopic structure

Abstract

The invention discloses a multi-color super-resolution imaging system applied to a 4pi microscopic structure. The multi-color super-resolution imaging system comprises a light source module, a sampletable, an upper microscope lens, a lower microscope lens, a first quarter-wave plate, a second quarter-wave plate, a first dichroscope, a second dichroscope, a first light path reflex matching unit, asecond light path reflex matching unit, a first deformable mirror, a second deformable mirror, a first Babinet's compensator, a second Babinet's compensator, a beam splitter cube, a third light pathreflex matching unit, a fourth light path reflex matching unit, a polarization beam splitter cube, an eleventh reflector, a twelfth reflector, a ninth lens, a tenth lens, a rectangular prism, a thirddichroscope, an eleventh lens, a twelfth lens, a first imaging device and a second imaging device. According to the system, the unimolecule positioning technology and crosstalk among different fluorescent molecules are utilized, and in combination with the 4pi microscopic technology, multi-color three-dimensional super-resolution imaging of whole cells is achieved.

Description

technical field [0001] The invention relates to the field of optical super-resolution microscopic imaging, in particular to a multi-color super-resolution imaging system applied to a 4pi microstructure. Background technique [0002] In the fields of life science, biomedicine, chemical science, material science, etc., imaging the target substance is an important research method. With the deepening of research, the size of the imaged target becomes smaller and smaller. However, the conventional wide-field microscope belongs to the far-field imaging method. Due to the limitation of the diffraction limit, the resolution is only 200nm, which is far from the ability to distinguish subcellular structures. Near-field imaging techniques, such as electron microscopes, scanning tunneling microscopes, atomic force microscopes, etc., can obtain ultra-high resolution of 0.1nm. High requirements, not suitable for live cell imaging and other reasons, limit the applicability of near-field i...

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Problems solved by technology

[0005] The present invention aims at the problems that the existing super-resolution microscopy system realizes three-dimensional imaging of biological samples, the resolution in the depth direction is low and limited to thin samples, and it can only realize two-color imaging and is prone to produce crosstalk and artifacts, etc., and proposes an application Multi-color super-resolution imaging system based on 4pi microstructure

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