The invention discloses a multi-color super-resolution imaging
system applied to a 4pi microscopic structure. The multi-color super-resolution imaging
system comprises a
light source module, a sampletable, an upper
microscope lens, a lower
microscope lens, a first quarter-wave plate, a second quarter-wave plate, a first dichroscope, a second dichroscope, a
first light path
reflex matching unit, asecond light path
reflex matching unit, a first
deformable mirror, a second
deformable mirror, a first Babinet's compensator, a second Babinet's compensator, a
beam splitter cube, a third light pathreflex matching unit, a fourth light path
reflex matching unit, a
polarization beam splitter cube, an eleventh reflector, a twelfth reflector, a ninth lens, a tenth lens, a rectangular
prism, a thirddichroscope, an eleventh lens, a twelfth lens, a first imaging device and a second imaging device. According to the
system, the unimolecule
positioning technology and
crosstalk among different fluorescent molecules are utilized, and in combination with the 4pi microscopic technology, multi-color three-dimensional super-resolution imaging of whole cells is achieved.