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Lactobacillus salivarius SNK-6 and application thereof

A technology of Lactobacillus salivarius and a preservation number, applied to Lactobacillus salivarius SNK-6 and its application field, can solve the problems of avoiding dark spots on eggs, unable to completely avoid dark spots on eggs, and insignificant effects, so as to avoid dark spots on eggs. Effect

Active Publication Date: 2018-10-02
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the effects of improving egg quality through the above methods are quite different, and there is no substantial or insignificant effect on reducing egg dark spots.
The effect of improving egg quality through genetic breeding is relatively slow, and the dark spot in eggs cannot be completely avoided by adjusting the nutritional content of feed; in addition, the use of traditional Chinese medicine to improve egg quality has a certain effect, but the effect is not significant, and there is no research on the use of traditional Chinese medicine to avoid egg dark spots. Related news about dark spots
However, there is no Lactobacillus salivarius isolated from the chicken intestine, and there is no related research and application report on avoiding egg dark spots through Lactobacillus salivarius

Method used

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  • Lactobacillus salivarius SNK-6 and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The isolation of embodiment 1 Lactobacillus salivarius SNK-6

[0020] First observe the dark spots of the eggs, find out the hens that produce eggs without dark spots or less dark spots, prepare special medium and 5 10cm plates; then prepare 2mL centrifuge tubes, add 1mL of the above special medium, and collect the hens with cotton swabs Put 1 g of fresh feces from the small intestine into the above-mentioned centrifuge tube containing the special medium, shake it 10 times, mix it and let it stand for 1 minute; then draw the upper layer solution into another 2mL centrifuge tube, supplement the medium to 1.5mL, shake it 10 times, Mix and let stand for 1 minute, repeat this operation once.

[0021] Then distribute 1mL of the upper culture medium to 5 plates, smear the plates, and incubate them in an anaerobic incubator at 42°C for 24 hours, pick out prominent pale white colonies, and observe them with an optical microscope after Gram’s staining, as shown in the attached ...

Embodiment 2

[0022] Embodiment 2 Lactobacillus salivarius SNK-6 raises laying hen test

[0023] New poplar black-feathered laying hens were selected for the feeding experiment and divided into two groups on average. One group of laying hens was raised according to a standardized feeding method; Make 1000mL of normal saline solution, spray it on the surface of the feed with a watering can after mechanical feeding, and repeat the spray once the next day. After continuous spraying of Lactobacillus salivarius SNK-6 strain for 3 weeks, the fresh eggs in the fourth week began to have no dark spots. Then spray the above-mentioned Lactobacillus salivarius SNK-6 bacterial agent once a week, and the fresh eggs will not produce dark spots. The egg dark spot incidence rate and the Hashgard unit result of test production are as shown in Table 1, as can be seen, compared with not adding Lactobacillus salivarius SNK-6 of the present invention, the present invention can avoid egg dark spot, and the egg T...

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Abstract

The invention discloses a lactobacillus salivarius SNK-6 and the application thereof, wherein the lactobacillus salivarius SNK-6 has a preservation number of CCTCC NO: M 2018044, has a 16S DNA sequence as shown in SEQ ID NO: 1, and is derived from chicken intestines. The lactobacillus salivarius SNK-6 is used for preventing dark spots on eggs and improving egg quality.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a Lactobacillus salivarius SNK-6 and an application thereof. Background technique [0002] At present, the main ways to improve egg quality are: using laying hens with good egg quality, feeding high-quality feed, and providing a controllable production environment that meets the needs of laying hens and a better egg storage environment. Studies have also shown that adding The ingredients of traditional Chinese medicine can change the composition of eggs. However, the effects of improving egg quality by the above methods vary greatly, and all have no substantial effect or insignificant effect on reducing egg dark spots. The effect of improving egg quality through genetic breeding is relatively slow, and the dark spot in eggs cannot be completely avoided by adjusting the nutritional content of feed; in addition, the use of traditional Chinese medicine to improve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K50/75A23K10/18A01K67/02C12R1/225
CPCA01K67/02A23K10/18A23K50/75C12N1/205C12R2001/225
Inventor 严华祥
Owner SHANGHAI ACAD OF AGRI SCI
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