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SNP marker relevant to ear ramulus number character of millet as well as detection primers for SNP marker and applications

A technology of labeling and millet, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, recombinant DNA technology, etc., and can solve the problems of unreported research on the development and application of SNP molecular markers.

Active Publication Date: 2018-10-12
ZHANGJIAKOU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the research on the QTL mapping and SNP markers related to the quantitative traits of millet is mainly focused on the development and utilization of SSR markers, but the development and application of SNP molecular markers using degenerate genome sequencing of population inbred lines to detect polymorphisms has not yet been reported.

Method used

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  • SNP marker relevant to ear ramulus number character of millet as well as detection primers for SNP marker and applications
  • SNP marker relevant to ear ramulus number character of millet as well as detection primers for SNP marker and applications
  • SNP marker relevant to ear ramulus number character of millet as well as detection primers for SNP marker and applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 High-throughput sequencing and SNP marker information analysis

[0034] In this example, the materials of the male parent "Ji Zhanggu No. 1" and the female parent "A2" of millet, the F1 generation materials of the two hybrids, and 441 materials of the F2 population of the 13th generation of selfing, that is, recombinant inbred lines (Recombinant inbred lines , abbreviated as RILs). Each material was planted in Zhangjiakou, Hebei, and the trait data such as yardage were recorded and sorted out. After the degenerate genome resequencing of each material, the alignment of the reference genome was completed, and the SNP marker was obtained. By analyzing the code number trait data, the associated SNP markers were obtained, and the SNP markers were verified by clonal sequencing.

[0035] In this example, the RADseq method was used to extract the genomic DNA of each sample individual, a total of 444 samples, including 441 RILs, 2 parents, and 1 F1 individual. The q...

Embodiment 2

[0058] Embodiment 2 SNP marker verification

[0059] According to the predicted bin marker and SNP site, compare the reference genome to obtain the gene sequence of the relevant region, select about 300 bp before and after the SNP site, design and develop SNP marker primers, and use the DNA of the paternal and maternal materials as templates for PCR amplification. Select the amplified products with normal primer amplification and the compounded predicted size of the PCR amplification products for gel cutting recovery and sequencing, and select the labeled primers with SNP site differences in the amplified gene sequences of the paternal and maternal materials. Using the F1 and F2 generation materials as templates, carry out PCR amplification of SNP marker primers and sequencing of the results, and select marker primers that are consistent with genotyping and phenotypic traits.

[0060] First, according to the sequencing results after PCR product recovery, the markers for the di...

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Abstract

The invention discloses an SNP marker relevant to the ear ramulus number character of millet as well as detection primers for the SNP marker and applications. The SNP marker relevant to the ear ramulus number character of millet provided by the invention is located in the bin markers from the 27578869bp to the 28178402bp of the second chromosome, and the SNP marker is in close linkage with the earramulus number of millet. The SNP marker relevant to the ear ramulus number character of millet provided by the invention can be used for the molecular mark assisted breeding of the ear ramulus number character of millet, through the detection of the SNP marker, the ear ramulus number of millet can be predicated, a scientific basis is provided for realizing the early identification of the ear ramulus number character of millet or screening breeding, and great theory and practice guiding significance is achieved for accelerating the genetic breeding or improvement process of the millet varieties. The primer pair for detecting the SNP marker provided by the invention can realize typing on the ear ramulus number of millet, and detect the differences of SNP expression.

Description

technical field [0001] The present application relates to the technical field of millet breeding, in particular to a SNP marker related to millet yardage traits, detection primers and applications thereof. Background technique [0002] my country is the origin of millet and the country with the largest cultivated area and the highest yield in the world, accounting for about 80% of the world's total. At the same time, my country is also the country with the largest number and diversity of millet genetic resources. [0003] Corn ears are composed of cobs and many grain yards. The yardage and grain number are important factors affecting the yield of millet. The study of the genes and their position functions related to the control of millet yardage, grain number and other yield traits is of great importance for guiding Millet genetics and breeding are of great significance. [0004] Single nucleotide polymorphism (singlenucleotide polymorphism, SNP) is a DNA sequence polymorp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 赵治海李双东王晓明张晓磊张耕耘史高雷魏玮张雅莉裴晶晶
Owner ZHANGJIAKOU ACAD OF AGRI SCI