Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Detection method for mutation screening of disease-causing genes of Parkinson's disease

A technology for Parkinson's disease and detection method, which is applied in the field of Parkinson's disease pathogenic gene mutation screening and detection, and can solve problems such as no cure.

Inactive Publication Date: 2018-10-16
XIANGYA HOSPITAL CENT SOUTH UNIV
View PDF2 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, the treatment of PD is mainly to treat the cause, and there is no cure

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0036] It should be noted that, in the case of no conflict, the embodiments of the present invention and the features in the embodiments can be combined with each other.

[0037] The present invention will be described in detail below in conjunction with examples.

[0038] To achieve the above object, the present invention provides the following technical solutions:

[0039] A method for screening and detecting Parkinson's disease-causing gene mutations, comprising the following steps:

[0040] (1) Add dd H2O to the synthesized MIPs probe to make a probe solution with a concentration of 100 μM, and store it at -20°C for later use;

[0041](2) Use a pipette to draw 1 μl of each probe solution in (1), put it into a new 1.5ml EP tube, mix well, and use a Marker pen on the tube wall to mark it as mixed-MIPs;

[0042] (3) Configure the MIPs probe phosphorylation activation system to activate mixed-MIPs;

[0043] The reaction system is as follows: 25 μl of mixed-MIPs, 3 μl of T4P...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a detection method for mutation screening of disease-causing genes of Parkinson's disease (PD). The detection method comprises the following steps: (1) adding dd H2O into a synthesized MIPs probe to prepare a probe solution with the concentration of 100mu M; (2) sucking 1mu l of the probe solution in step (1) by utilizing a pipettor; then putting the probe solution into a 1.5ml new EP pipe; sufficiently and uniformly mixing; marking a pipe wall as mixed-MIPs by utilizing a Marker pen; (3) preparing an MIPs probe phosphorylation activation system and carrying out activation on the mixed-MIPs; (4) diluting a mixed-MIPs probe; (5) preparing an MIPs target capturing reaction system; (6) preparing an MIPs enzyme digestion reaction system; (7) carrying out PCR (PolymeraseChain Reaction) amplification reaction; (8) carrying out polyacrylamide gel electrophoresis and magnetic bead purification; (9) carrying out high-throughput sequencing on a mixed library; (10) carrying out Sanger sequencing verification. By adopting the detection method for the mutation screening of the disease-causing genes of the Parkinson's disease, the mutation screening of the 18 PD disease-causing genes of the Parkinson's disease can be finished, and the detection method has important significance on researches of super-early diagnosis and PD pathogenesis of potential PD patients.

Description

technical field [0001] The invention relates to the technical field of screening and detection of Parkinson's disease-causing gene mutations, in particular to a method for screening and detecting Parkinson's disease-causing gene mutations. Background technique [0002] Parkinson's disease (Parkinson's Disease, PD) is a common neurodegenerative disease (Neurodegenerative diseases), with a high prevalence rate. Epidemiological surveys at home and abroad show that the prevalence rate of PD in the elderly population over 65 years old is about 1 to 2%, and the prevalence rate of the elderly over 80 years old is as high as 4%. There are about 2.5 million PD patients in my country. With the aging of the population and the extension of life expectancy in my country, it is estimated that by 2030, there will be about 5 million PD patients in China, accounting for 57% of the total number of PD patients in the world. The onset of PD patients is insidious, and the early clinical manifes...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6858
CPCC12Q1/6858C12Q2521/301C12Q2531/113C12Q2535/122
Inventor 郭纪锋唐北沙严新翔
Owner XIANGYA HOSPITAL CENT SOUTH UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com