A set of sturgeon SNP molecular markers, evaluation methods for sturgeon seed production and genetic evaluation methods
A molecular marker, sturgeon technology, applied in the field of genetic evaluation and sturgeon SNP molecular marker, can solve the problems of genetic diversity analysis, breeding problems of fine varieties, scarcity of microsatellite markers, difficulties in nuclear gene analysis and research, etc. The cost of genetic testing, the wide range of applications, and the effect of avoiding cumbersomeness
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[0061] Genetic Evaluation of 18 Candidate Parents of Chinese Sturgeon
[0062] 1. Genomic DNA Extraction
[0063] Blood was collected from 18 candidate broodstocks of Chinese sturgeon, including 12 females (A01-A12) and 6 males (B01-B06). Blood was anticoagulated with EDTA and stored at 4°C. Take 20 μL of blood, use the Marine Animal Genomic DNA Extraction Kit (Tiangen DP324-03), extract genomic DNA according to the instructions, dissolve it in 100 μL of sterilized deionized water, and measure the concentration with an ultraviolet spectrophotometer.
[0064] 2. SNP typing
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