A set of sturgeon SNP molecular markers, evaluation methods for sturgeon seed production and genetic evaluation methods
A molecular marker, sturgeon technology, applied in the field of genetic evaluation and sturgeon SNP molecular marker, can solve the problems of genetic diversity analysis, breeding problems of fine varieties, scarcity of microsatellite markers, difficulties in nuclear gene analysis and research, etc. The cost of genetic testing, the wide range of applications, and the effect of avoiding cumbersomeness
Active Publication Date: 2021-07-13
CHINESE ACAD OF FISHERY SCI
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Problems solved by technology
However, due to the polyploidy of the sturgeon genome and the ploidy differences among different species, it brings difficulties to the analysis of nuclear genes, and the number of microsatellite markers developed in sturgeon species is also relatively rare compared with other model aquatic organisms , causing great troubles to vigorously develop sturgeon molecular markers, analysis of sturgeon genetic diversity, and breeding of improved species
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Embodiment 1
[0061] Genetic Evaluation of 18 Candidate Parents of Chinese Sturgeon
[0062] 1. Genomic DNA Extraction
[0063] Blood was collected from 18 candidate broodstocks of Chinese sturgeon, including 12 females (A01-A12) and 6 males (B01-B06). Blood was anticoagulated with EDTA and stored at 4°C. Take 20 μL of blood, use the Marine Animal Genomic DNA Extraction Kit (Tiangen DP324-03), extract genomic DNA according to the instructions, dissolve it in 100 μL of sterilized deionized water, and measure the concentration with an ultraviolet spectrophotometer.
[0064] 2. SNP typing
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The invention provides a group of sturgeon SNP molecular markers. The invention also provides a method for genetic evaluation of the sturgeon candidate parents. The present invention also provides a method for evaluating seed production parents among the sturgeon candidate parents, so as to select excellent seed production parents under the premise of abundant genetic diversity of the sturgeon population; or, when the genetic diversity of the sturgeon population is low In the case of the introduction of foreign parents. The invention also provides a method for assessing the pedigree of a sturgeon individual. According to the identification result, the genetic relationship between a pair of sturgeon parents and their juvenile sturgeon can be determined. The invention also provides a method for evaluating the genetics of the sturgeon population, which determines the genetic relationship between the sturgeon parents and the sturgeon juveniles in the sturgeon population according to the evaluation result. The combination of 96 SNP molecular markers provided by the invention can not only reflect the genetic combination information between different chromosomes, but also reflect the genetic linkage information on the same chromosome, and can fully meet the requirements of genetic detection in the seed production process.
Description
technical field [0001] The invention relates to the technical field of aquatic organisms, in particular to a group of sturgeon SNP molecular markers, a sturgeon seed production evaluation method and a genetic evaluation method. Background technique [0002] Sturgeon is a general term for fishes of the order Acipenseriformes, belonging to the class Osteichthyes, the subclass Actinopterygii, and the general order Chondrostei. Sturgeon artificial breeding began in the 1980s, but the scale of the industry was once small. Since the beginning of the 21st century, my country's sturgeon aquaculture industry has entered a period of rapid development. The annual output has increased from 9,400 tons in 2003 to 89,800 tons in 2016. The breeding areas cover Beijing, Heilongjiang, Guangdong, Shandong, Yunnan and other 20 In many provinces, the product form has also developed from a single model dominated by caviar in the past to a diversified direction of caviar, fish meat, fish skin and ...
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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156
Inventor 赵紫霞江炎亮许建张研
Owner CHINESE ACAD OF FISHERY SCI