An array type line scanning fluorescence microscopy imaging device

Abstract

The invention discloses an array type line scanning fluorescence microscopic imaging apparatus, which comprises a three-dimensional translation table and two imaging structures, wherein the three-dimensional translation table is used for moving a sample in a X direction, a Y direction and a Z direction, each imaging structure is used for carrying out line scanning imaging on the one imaging stripof the sample surface in an inclined detection manner in one scanning so as to achieve the multi-line simultaneous scanning imaging at the same plane in the form of an array, the imaging structure comprises an excitation light module and a detection module, the excitation light module is used for generating an excitation line light spot having a specified width and obtained by shaping with a laserbeam, the divergence angle and the inclination angle of the laser beam can be adjusted, and the detection module is used for concentrating the excitation line light spot on the sample surface to excite the sample to generate a fluorescent signal, and carrying out detection imaging on the fluorescent signal generated by the sample. According to the present invention, with the array type line scanning fluorescence microscopic imaging apparatus, the imaging speed of the line scanning imaging can be effectively improved, and the adjustment difficulty of the system can be reduced.

Description

technical field [0001] The invention belongs to the field of design and manufacture of fluorescence microscope instruments, and more specifically relates to an array type line scanning fluorescence microscope imaging device. Background technique [0002] In the field of bioengineering technology, confocal microscope is one of the widely used instruments in the field of biological research and medical research. [0003] The traditional laser scanning confocal microscope improves the clarity of the image by enhancing the contrast between the observed object and the background, thereby indirectly improving the resolution of the presentation. The traditional confocal microscope uses a point light source as the excitation light of the fluorescent sample, and uses a photomultiplier tube for point detection. In order to improve the contrast of the image, the confocal microscope designs a small hole conjugated with the point excitation light source on the image plane. The small hol...

AI Technical Summary

Problems solved by technology

[0004] Since the traditional confocal microscope uses a small hole conjugated with the excitation point light source to suppress the background light source, the position adjustment of the small hole and the processing accuracy of the small hole will affect the imaging quality of the confocal microscope

Limited by the processing technology and the difficulty of adjusting the small holes, the overall construction and adjustment of the confocal microscope are very difficult

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