Supercharge Your Innovation With Domain-Expert AI Agents!

Method for determinating Advantame in food

A technology of food and Amity, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems such as the lack of relevant literature on Advantam, and achieve the effect of meeting the needs of daily testing work, low matrix interference, and fast analysis speed

Inactive Publication Date: 2018-10-19
大连市食品检验所
View PDF4 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although my country has approved the use of advantame, there is no relevant detection standard for advantame in food, and there are very few relevant documents on the detection of advantame at home and abroad, mainly for toxicology-related research

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for determinating Advantame in food
  • Method for determinating Advantame in food
  • Method for determinating Advantame in food

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] (1) Liquid-liquid extraction: Weigh 2.0 g of the sample, place it in a 20 mL polypropylene centrifuge tube, add 5 mL of 20% acetonitrile solution, vortex for 30 s, sonicate for 5 min, centrifuge at 8000 r / min for 5 min, and transfer the supernatant to 10 mL volumetric flask. Dilute to volume with 20% acetonitrile solution, filter membrane (0.22 μm, organic phase) for milk-free samples, to be analyzed; milk-containing samples, to be purified.

[0072] (2) Liquid-solid extraction: Weigh 2.0 g of sample, place it in a 20 mL polypropylene centrifuge tube, add 5 mL of 20% acetonitrile solution, vortex for 30 s, sonicate for 5 min, centrifuge at 8000 r / min for 5 min, and transfer the supernatant to 10 mL volumetric flask. Dilute to volume with 20% acetonitrile solution, filter membrane (0.22 μm, organic phase) without milk samples, to be analyzed; milk samples, to be purified.

[0073] (3) Liquid-liquid distribution and purification of the sample to be purified: Add 2 mL of...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for determinating Advantame in food. The method comprises the following steps: performing liquid-liquid (liquid-solid) extraction / ultrasonic-assisted extraction: adding an extracting solvent into a food sample for extraction, performing whirling oscillation, performing ultrasonic-assisted extraction, then centrifuging, and taking 1mL of a supernatant, wherein for amilk-free sample, filtration by a microporous filtration membrane is performed for analysis, and for a milk-containing sample, liquid-liquid distribution purification is performed; preparing a seriesof standard working solutions: taking an Advantame sample, dissolving in methanol, fixing the volume in a 10mL volumetric flask to obtain a standard stock solution, then diluting with methanol to obtain a standard intermediate solution, separately sucking the standard intermediate solution, and diluting with an extracting solution to obtain the series of standard working solutions containing theAdvantame with the concentrations of 0.15ng / mL, 0.3ng / mL, 0.6ng / mL, 1.0ng / mL, 2.0ng / mL and 5.0ng / mL; performing qualitative and quantitative analysis: introducing the series of standard working solutions and a solution to be analyzed into a liquid chromatograph tandem mass spectrometer, monitoring in a multi-reaction monitoring mode, and quantifying by an external standard method.

Description

technical field [0001] The invention relates to a method for determining the sweetener Advantame in food, specifically a method for determining Advantame in food based on liquid-liquid (solid) extraction-liquid chromatography-tandem mass spectrometry, which belongs to food-oriented The field of monitoring technology mainly involves flavored fermented milk, frozen drinks, candy, tea drinks, coffee drinks, plant drinks, jelly, processed fruits, etc., specifically related to positive ion mode determination based on liquid-liquid (solid) extraction-liquid chromatography-tandem mass spectrometry method. Background technique [0002] Advantame (N-[N-[3-(3-hydroxy-4-methoxyphenyl)propyl-L-a-aspartyl]-L-phenylalanine-1-methyl ester 〕) is an ultra-high sweetener with 20,000 times sweetness. It is a derivative of aspartame. Its chemical structure is similar to neotame. It has good water solubility and high heat resistance temperature, and is suitable for high-temperature processing p...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N30/88G01N30/06
CPCG01N30/06G01N30/88G01N2030/047G01N2030/062G01N2030/884
Inventor 曲宝成戴学东姜俊毛希琴李鹏勇艳华刘畅王硕
Owner 大连市食品检验所
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com