Microalgae monitoring device and microalgae monitoring method
A monitoring device, technology of microalgae, applied in the field of environment
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no. 1 Embodiment approach
[0094] like figure 1As shown, the observation apparatus for microalgae according to the first embodiment includes: a flow cell 40 for flowing a fluid containing microalgae; an excitation light source 10 for irradiating the flow cell 40 with excitation light; and a first fluorescence detector 102A , which detects autofluorescence generated by the lipids of the respective microalgae irradiated with excitation light; a scattered light detector 105 which detects the scattered light generated by each of the microalgae; and a recording section 301 which records the detected light in time series Autofluorescence of lipids and intensity of scattered light. The recording unit 301 is included in, for example, a central processing unit (CPU) 300 . The lipids contained in microalgae are also known as oil bodies. The fluid flowing in the flow cell 40 may be liquid or gas. Hereinafter, an example in which the fluid is a liquid will be described. In addition, lipids include lipids that a...
no. 2 Embodiment approach
[0143] like Figure 16 As shown, the CPU 300 of the microalgae observation apparatus according to the second embodiment is further provided with the ability to compare the intensity of scattered light, the intensity of autofluorescence by lipids, and the intensity of autofluorescence by chloroplasts that are simultaneously detected. of the comparison section 307.
[0144] The comparison unit 307 calculates, for example, the ratio of the intensity of the autofluorescence emitted by the lipid of the microalgae to the intensity of the scattered light. The comparison unit 307 may normalize the value of the intensity of the scattered light to 100 or the like to calculate the ratio of the intensity of the autofluorescence emitted by the lipid of the microalgae to the intensity of the normalized scattered light.
[0145] Further, the comparison unit 307 calculates, for example, a ratio of the intensity of autofluorescence emitted by the chloroplast of the microalgae to the intensity...
reference example 1
[0155] Chlorella vulgaris Beijerinck (NIES-2170) was purchased from the Microbial Systems Conservation Facility of the National Institute of Environmental Research and Development Corporation. After that, Chlorella was cultured in a liquid C medium in a constant temperature chamber at 25°C. During the culture, the tube containing Chlorella and liquid C medium was shaken at 100 rpm. In addition, in the culturing process, the fluorescent lamps of daylight color were repeatedly turned on for 10 hours and turned off for 14 hours in the thermostatic chamber according to the recommended culturing conditions of the distributor.
[0156] 10 μL of liquid C medium containing the cultured Chlorella that was not fluorescently stained was dropped onto a glass slide and covered with a cover slip. Next, the chlorella without fluorescence staining was photographed with a UIS microscope manufactured by Olympus Co., Ltd. Figure 19 Transmission microscope images shown.
[0157] Thereafter, t...
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