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Temporary Preservation and Vegetative Propagation of Male Sterile Plants of Cabbage

A technology of male sterility and asexual reproduction, applied in botany equipment and methods, horticultural methods, vegetative reproduction, etc., can solve the problems of long time, tediousness, heavy workload, etc., and achieve high regeneration rate, high survival rate, rooting high rate effect

Active Publication Date: 2021-10-01
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] 1. Self-fertilization, mainly suitable for partial sterile and functional sterile plants is invalid for 100% sterile plants; 2. Self-fertilization after hybridization, F1 after hybridization with fertile sister plants of the same variety, and then self-fertilization is expected to be effective A certain proportion of sterile plants appear in F2, which takes a long time and requires a lot of work
3. Sterile plants obtained by distant hybridization can be backcrossed with both parents. For the recessive gene controlling male sterility, BC1 will disappear, and it is also expected to self-seed to separate sterile plants, which is cumbersome and risky
The mother plant of cabbage can only know whether it is fertile after flowering. At this time, it is different from the vegetative growth stage. The regeneration rate of asexual reproduction is low, and the survival rate of cuttings of leaves and axillary buds is low, which needs to be improved.

Method used

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  • Temporary Preservation and Vegetative Propagation of Male Sterile Plants of Cabbage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Temporary preservation and asexual propagation method of akamu male infertility

[0025] Glycascular branches

[0026] The original male infertile plants that temporarily discovered.

[0027] 1.1 When the casbech is blooming, it is found that there is a male infertility plant, and the plants are adjusted, take a few times, first remove the main bone flowers; sequentially remove the three-stage branches, the secondary side branch, leave some blade photosynthetic growth. After 7 days, the buds of the three-stage branches began to appear, and timely remove.

[0028] 1.2 1 / 3 of the lower portion of the mother plant, remove the leaves, expose the outer stems, water moisturizing, and shake it for 3 days with black plastic.

[0029] 1.3 Due to the destruction of the top of the flower branch, there will be some stems from the outer root of the mother. It has been gradually growing from the orthoperometrics, and about three batches by large to small axillary buds. .

[003...

Embodiment 2

[0039] Example 2 Temporary preservation and asexual propagation method of the brusselsum male infertility

[0040] Glycascular branches

[0041] The original male infertile plants that temporarily discovered.

[0042] 1.1 When the casbech is blooming, it is found that there is a male infertility plant, and the plants are adjusted, take a few times, first remove the main bone flowers; sequentially remove the three-stage branches, the secondary side branch, leave some blade photosynthetic growth. After 10 days, the buds of the three-stage branches began to appear, and timely remove.

[0043] 1.2 At 1 / 3 of the lower portion of the mother, remove the leaves, expose the outer stems and leaves, water moisturizing, shade 3-5 days with black plastic.

[0044] 1.3 Due to the destruction of the top of the flower branch, there will be some stems from the outer root of the mother. It has been gradually growing from the orthoperometrics, and about three batches by large to small axillary buds....

Embodiment 3

[0055] Example 3 Temporary preservation and asexual breeding method of akamus male infertility

[0056] Glycascular branches

[0057] The original male infertile plants that temporarily discovered.

[0058] 1.1 When the casbech is blooming, it is found that there is a male infertility plant, and the plants are adjusted, take a few times, first remove the main bone flowers; sequentially remove the three-stage branches, the secondary side branch, leave some blade photosynthetic growth. After 8 days, the buds of the three-stage branches began to appear, and timely remove.

[0059] 1.2 At 1 / 3 of the lower portion of the mother, remove the leaves, expose the outer stems and leaves, water moisturizing, shade 3-5 days with black plastic.

[0060] 1.3 Due to the destruction of the top of the flower branch, there will be some stems from the outer root of the mother. It has been gradually growing from the orthoperometrics, and about three batches by large to small axillary buds. .

[0061] ...

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Abstract

The invention discloses a method for temporary preservation and asexual reproduction of male sterile cabbage plants, comprising: (1) destroying the apex dominance of the male sterile cabbage plants, and promoting the growth of axillary buds from the outer rhizome leaf scars of the mother plant; (2) The axillary buds are buckled from the outer rhizome and ensure that the larger axillary buds have a piece of rhizome pith tissue; (3) the larger axillary buds with the rhizome pith tissue are asexually propagated to obtain a new generation of plants; The axillary buds are asexually propagated by tissue culture to obtain rooted seedlings, and the rooted seedlings are trained and then moved to the open field for cultivation. The method for temporary preservation and asexual reproduction of cabbage male sterile plants of the present invention promotes the growth of axillary buds from the leaf scars of the outer roots and stems of the mother plant by breaking the dominance of the apex, and adopts cuttings for the grown larger axillary buds to carry out asexual reproduction, and the survival rate of cuttings is improved. High; for smaller axillary buds, tissue culture is used for vegetative propagation and the best medium for axillary bud rooting is optimized, and the regeneration rate is high.

Description

Technical field [0001] The present invention relates to the preservation and propagation method of vegetable male stress, in particular, to temporary preservation and asexual propagation methods of the bronuch male infertile, belonging to the temporary preservation and reproductive field of the kale male infertility. Background technique [0002] The cabbage has a significant hybrid advantage, hybrid one generation (F 1 ) Is better than double princes or a parent in a certain character or a plurality of traits. Using hybrid advantages, it can not only improve the yield and quality of the cabbage variety, but also improve its disease resistance, anti-counterpostation, has become the main research direction of current Chinese cabbage varieties. Variety breeding and hybridism can be used to expand the use of hybrid advantages, but also the propagation of hybrids, but it is relatively easy to reproduce, avoiding the labor-to-life, which saves a lot of manpower and material power, the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G2/10A01G7/06
CPCA01G7/06A01H4/001A01H4/008A01G2/10
Inventor 王超张晓烜许蕊仙张淑波
Owner NORTHEAST AGRICULTURAL UNIVERSITY