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A kind of lyoprotectant for oral attenuated lyophilized vaccine against swine fever and its application

A freeze-dried protective agent, swine fever mouth technology, applied in vaccines, veterinary vaccines, antiviral agents, etc., can solve problems such as unfavorable growth of pigs

Inactive Publication Date: 2021-05-07
张志刚
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the CSF vaccines used in my country mainly include injection-type vaccines such as the attenuated strain of CSF, rabbit spleen lymphoid tissue vaccine, and suckling pig kidney cell vaccine. They are mainly injected subcutaneously or intramuscularly. Only holding in place will cause the pigs to stress, which is not good for the growth of the pigs

Method used

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  • A kind of lyoprotectant for oral attenuated lyophilized vaccine against swine fever and its application
  • A kind of lyoprotectant for oral attenuated lyophilized vaccine against swine fever and its application
  • A kind of lyoprotectant for oral attenuated lyophilized vaccine against swine fever and its application

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Embodiment 1

[0039] Embodiment 1: a kind of oral attenuated lyophilized vaccine for classical swine fever, comprising attenuated classical swine fever virus, lyoprotectant and vaccine diluent, is characterized in that, comprises mucosa immune adjuvant and immunopotentiator in the described vaccine diluent, so The mucosal immune adjuvant includes propolis extract, levamisole and carbomer, and the immune enhancer includes astragalus polysaccharide and polygonatum polysaccharide.

[0040] The lyoprotectant includes 3% bovine serum albumin BSA, 2% mannitol, 2% glycine, 5% lactose and 88% deionized water in mass percent.

[0041] Each liter of the vaccine diluent contains: 130mM sodium chloride, 3.0mM potassium chloride, 10mM disodium hydrogen phosphate, 2mM sodium dihydrogen phosphate, 1.2g carbomer, 2.4g levamisole, 4g propolis extract, 5g Astragalus polysaccharide, Polygonatum Polysaccharide 2g.

[0042] The preparation method of the vaccine diluent is: dissolving sodium chloride, potassium...

Embodiment 2

[0052] Embodiment 2: a kind of oral attenuated lyophilized vaccine of classical swine fever, comprises attenuated classical swine fever virus, lyoprotectant and vaccine diluent, it is characterized in that, comprises mucosa immune adjuvant and immune enhancer in the described vaccine diluent, so The mucosal immune adjuvant includes propolis extract, levamisole and carbomer, and the immune enhancer includes astragalus polysaccharide and polygonatum polysaccharide.

[0053] The lyoprotectant includes 3% bovine serum albumin BSA, 2% mannitol, 2% glycine, 6% lactose and 87% deionized water in mass percent.

[0054] Each liter of the vaccine diluent contains: 130mM sodium chloride, 3.0mM potassium chloride, 10mM disodium hydrogen phosphate, 2mM sodium dihydrogen phosphate, carbomer 1.5g, levamisole 3.0g, propolis extract 2g , 3g of Astragalus Polysaccharide, 1.5g of Polygonatum polysaccharide.

[0055] The preparation method of the vaccine diluent is: dissolving sodium chloride, p...

Embodiment 3

[0065] Embodiment 3: a kind of oral attenuated lyophilized vaccine for classical swine fever, comprising attenuated classical swine fever virus, lyoprotectant and vaccine diluent, is characterized in that, comprises mucosa immune adjuvant and immunopotentiator in the described vaccine diluent, so The mucosal immune adjuvant includes propolis extract, levamisole and carbomer, and the immune enhancer includes astragalus polysaccharide and polygonatum polysaccharide.

[0066] The lyoprotectant includes 3% bovine serum albumin BSA, 2% mannitol, 2% glycine, 5% lactose and 88% deionized water in mass percent.

[0067] Each liter of the vaccine diluent contains: 130mM sodium chloride, 3.0mM potassium chloride, 10mM disodium hydrogen phosphate, 2mM sodium dihydrogen phosphate, 1.4g carbomer, 2.8g levamisole, 3g propolis extract, 2g Astragalus polysaccharide, Polygonatum Polysaccharide 1g.

[0068] The preparation method of the vaccine diluent is: dissolving sodium chloride, potassium...

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Abstract

The invention relates to a lyoprotectant for oral attenuated lyophilized vaccine against swine fever and its application. The lyoprotectant includes 3% bovine serum albumin BSA, 2% mannitol, and 2% Glycine, 5‑6% lactose, balance water. The present invention uses the attenuated strain of classical swine fever as the seed virus, and prepares an oral attenuated freeze-dried vaccine of classical swine fever with good immune effect through the improvement of the freeze-dried protective agent and the vaccine diluent. Complete protection with higher antibody titers, better / equal immune effect with commercially available injection vaccines, and easy to use.

Description

Technical field: [0001] The invention belongs to the field of biotechnology, and in particular relates to a freeze-drying protectant for an oral attenuated freeze-dried vaccine against swine fever and its application. Background technique: [0002] Classical swine fever (CSF) is an acute septicemia and highly contagious disease of pigs caused by classical swine fever virus (CSFV), characterized by high fever and diffuse hemorrhage in whole body tissues Symptoms and pathological features. CSF once caused great economic losses to the swine industry all over the world. The World Organization for Animal Health stipulated it as an animal infectious disease that must be reported. my country has also designated swine fever as a severe infectious disease. animal diseases, and diseases that require urgent, strict and compulsory prevention. Although some countries have successfully decontaminated CSFV, it still occurs occasionally in most pig farming areas of the world, distributed i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/19A61K47/42A61K47/26A61K47/18A61K39/187A61P31/14
CPCA61K9/19A61K39/12A61K47/183A61K47/26A61K47/42A61K2039/5254A61K2039/542A61K2039/552A61K2039/55511A61K2039/55583A61K2039/55588A61P31/14C12N2770/24334
Inventor 胡江锋张志刚
Owner 张志刚