Method for Enrichment and Culture of Microorganisms in River Sediment Surface Water
A technology of enrichment culture and microorganisms, which is applied in the field of enrichment culture of microorganisms in the surface water of river sediments, can solve the problems such as the inability to clarify the medium formula
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Embodiment 1
[0021] A method for enriching and cultivating microorganisms in the surface water of river bottom mud, using an enrichment device for enrichment and cultivation, the enrichment device comprising an outer culture box 1, a nutrition tube 2 and a culture bag 3 that are sequentially set from outside to inside , the upper end and the lower end of the outer cultivation box 1 are provided with a liquid circulation port 11, the nutrition tube 2 is a double-layer casing, and several filter holes are provided on the inner and outer tube walls of the double-layer casing; The outer culture box 1 is used to hold the liquid culture medium for enrichment culture; the inner pipe wall and the outer pipe wall of the double-layer casing are used to hold river sediment, and the double-layer casing is The filter holes do not allow sand to pass through, but allow other nutrients such as inorganic salts, amino acids, and proteins to pass through; Allow microbial cells to pass through, but allow othe...
Embodiment 2
[0023] A method for enriching and cultivating microorganisms in the surface water of river bottom mud, which is basically the same as the method in Example 1, the difference is that the nutrition tube 2 is formed by two filter bags with different diameters, and the filter sleeve The diameter of the filter hole is 2mm, which allows the passage of small molecules and microbial cells, but does not allow the passage of small particles of sediment; after the enrichment culture, take out the culture bag 3 and soak it in the buffer solution, the buffer solution is 0.1mol / L, pH6.8 The PBS buffer solution was changed every 1 h, and the culture bag 3 was taken out after 4 times, and the liquid in the culture bag 3 was collected to obtain the enriched culture.
Embodiment 3
[0025] A method for enriching and cultivating microorganisms in the surface water of river bottom mud, which is basically the same as the method in Example 1, the difference is that the nutrition tube 2 is formed by two filter bags with different diameters, and the filter sleeve The diameter of the filter hole is 3 mm, which allows small molecules and microbial cells to pass through, but does not allow small particles of sediment to pass through; after the enrichment culture, take out the culture bag 3 and soak it in the buffer solution, which is double distilled water, and change the buffer every 1 hour After 4 times, the culture bag 3 was taken out, and the liquid in the culture bag 3 was collected to obtain the enriched culture. It should be noted that, in this embodiment, the gap between the nutrition tube 2 and the inner tube of the culture bag 3 is filled with the enriched culture liquid medium.
[0026] Below we take the culture method of concrete bacterium as example, ...
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