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Extraction method of soybean liposome with strong oxidization stability

A technology of soybean oil body and extraction method, which is applied in the directions of fat oil/fat production, fat oil/fat refining, and fat production, etc., can solve problems such as solvent residues, etc., and achieve the effects of good integrity, safe and non-toxic method, and easy operation.

Inactive Publication Date: 2018-11-02
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problem of solvent residue in the existing oil body extraction process, and to provide a method for extracting soybean oil body with strong oxidation stability. The method uses water as a medium to extract soybean oil body, and the extracted Soybean oil body has strong oxidation stability and can be directly added to food as a natural emulsifier, avoiding the process of extracting vegetable oil through solvents. The process is simple, safe and reliable, and has certain advantages in the application of food industry

Method used

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  • Extraction method of soybean liposome with strong oxidization stability
  • Extraction method of soybean liposome with strong oxidization stability
  • Extraction method of soybean liposome with strong oxidization stability

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specific Embodiment approach 1

[0026] Embodiment 1: This embodiment describes a method for extracting soybean oil bodies with strong oxidation stability, using soybean seeds as raw materials, and extracting them by soaking, grinding, filtering, centrifuging, mixing, homogenizing and other processes , the specific steps of the method are as follows:

[0027] Step 1: Soaking: Soak 90.00±1.00 g of soybean seeds in 800~850 mL of deionized water for 18~24 h at 4°C;

[0028] Step 2: Grinding: Wash the full-grained seeds soaked in step 1 with deionized water twice, then add them to a pulverizer (HK-02 pulverizer, Guangzhou Hongxing Machinery Co., Ltd.) for grinding to obtain a homogenate thing;

[0029] Step 3: Filtration: the homogenate of step 2 is filtered through four layers of gauze;

[0030] Step 4: Mixing: Collect the filtrate, add sucrose to the filtrate, and mix thoroughly in an ice-water bath (the default temperature is 0°C), wherein the mass ratio of sucrose to filtrate is 1:4;

[0031] Step Five: Se...

specific Embodiment approach 2

[0038] Embodiment 2: In the method for extracting soybean oil bodies with strong oxidation stability described in Embodiment 1, in step 2, the grinding rotation speed is 14000-16000 rpm, and the grinding time is 4-5 min.

specific Embodiment approach 3

[0039] Embodiment 3: In the method for extracting soybean oil bodies with strong oxidation stability described in Embodiment 1, in step 5 (4), the mass ratio of oil bodies to deionized water is 1:8.

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Abstract

The invention discloses an extraction method of soybean liposome with strong oxidization stability, and belongs to the technical field of health food or biology. The method comprises the following steps: performing soaking: soaking soybean seeds with deionized water; performing grinding: cleaning the soaked seeds with the deionized water, and then grinding the seeds to obtain a homogenate substance; performing filtering: filtering the homogenate substance through four layers of gauze; performing mixing: collecting filtrate, adding saccharose into the filtrate, and fully mixing the filtrate with the saccharose in an iced water bath; performing separation: separating the mixture with a centrifugal tube; and collecting all the liposome on the upper layer of the centrifugal tube for homogenization, thus obtaining the liposome. The extraction method has the advantages that the method for extracting the liposome by water serving as a medium is safe and nontoxic and is easy and convenient tooperate; the soybean liposome with relatively high intactness can be obtained and applied to food favorably and has an extremely good effect in the aspects of health product processing, the biologicaltechnical field and the like. The soybean liposome extracted by the extraction method can also be added into any other food or animal feed products in a dried and rehydrated manner, such as mayonnaise.

Description

technical field [0001] The invention belongs to the field of health food or biotechnology, and in particular relates to a method for extracting soybean oil bodies with strong oxidation stability. Background technique [0002] Oil bodies (Oil Bodies, OBs) are important organs for storing fat in oil crop seeds, which are composed of neutral lipids, phospholipids surrounding neutral lipids, and proteins embedded in the phospholipid layer. The particle size of soybean oil body is 0.2~0.5 μm, the interior is liquid triacylglycerol, and the exterior is a semi-unit membrane composed of a single layer of phospholipid molecules and their mosaic protein (oleosin), which has a unique structure and composition and is easy to extract , more stable characteristics. [0003] In recent years, soybean oleosomes have received more and more attention, which can be used as a natural and healthy oil-protein combination in food, medicine, and cosmetics, without the need for emulsification and ho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/00C11B1/04C11B3/00A23K20/158A23L27/60A23L29/00
CPCA23K20/158A23L27/60A23L29/04C11B1/00C11B1/04C11B3/001
Inventor 侯俊财姜瞻梅姜梦婷周鑫刘志静
Owner NORTHEAST AGRICULTURAL UNIVERSITY