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Marker and application of a gene related to chlorophyll content in wheat

A chlorophyll content, wheat technology, applied in the biological field, can solve the problems of slow application progress and great influence

Active Publication Date: 2022-05-17
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the measurement of physiological traits is greatly affected by environmental conditions, and the application in the improvement of drought resistance of wheat is progressing slowly

Method used

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  • Marker and application of a gene related to chlorophyll content in wheat
  • Marker and application of a gene related to chlorophyll content in wheat
  • Marker and application of a gene related to chlorophyll content in wheat

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1, the SNP marker of the gene related to wheat chlorophyll content

[0045] 1. Green phenotype survey

[0046] Use the SPAD-502 chlorophyll meter (Minolta Camera Co., Japan) to measure the middle sections of the flag leaf, the second leaf and the third leaf of the DH population, avoiding the middle veins. The plants with the same growth potential in the plot were selected for measurement, and the average value of each leaf position was measured 5 times. The measurement period is the flowering period and every 7 days after flowering until maturity, a total of 5 periods, which are recorded as S1-S5.

[0047] 2. Primer acquisition and marker analysis

[0048] SNP (Single Nucleotide Polymorphism) markers were detected by Illumina SNP genotyping at CapitalBio Corporation, Beijing, China; http: / / bioservices.capitalbio.com, mainly divided into the following steps: 1 ) Amplify the whole genome of the genomic DNA of the wheat variety to be tested; 2) cut the amplif...

Embodiment 2

[0078] Example 2, the application of SNP AX94573388 site in the detection of wheat chlorophyll content

[0079] Fifteen offspring varieties and parents of Feiyangmai 16 / Zhongmai 895DH population were selected. Among them, 15 non-Yangmai 16 / Zhongmai 895DH population progeny varieties were used as the test group, Zhongmai 895 and Yangmai 16 as the control group; including Afu, CA1055, CA1133, Lunxuan 987, Jingdong 8, Qinnong 731, Wan Mai 52, Jining 16, Jimai 21, Lumai 15, Luomai 21, Chuannong 52, Mianyang 26, Ningmai 9, Zhenmai 6.

[0080] 1. Detection of chlorophyll content in flag leaves of different varieties of wheat in late growth period

[0081] The identification of chlorophyll content in leaves of wheat varieties was carried out in the summer of 2017 at the Dongbian Farm of the Institute of Crop Science, Chinese Academy of Agricultural Sciences. A completely randomized block design was adopted, with a row length of 1.5m, a row spacing of 25cm, and a plant spacing of 10cm...

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Abstract

The invention discloses a marker and application of a gene related to chlorophyll content in wheat. The present invention provides the application of the genotype detection material of SNP AX94573388 site on wheat chromosome 4BL in the identification or auxiliary identification of wheat chlorophyll content; said SNP AX94573388 site is the nucleoside shown in sequence 4 located on wheat chromosome 4BL The 36th position of the acid sequence. The invention obtains a wheat chlorophyll content main effect gene site QChl.caas-4BL (752cM) and the site's auxiliary screening SNP site of wheat chlorophyll content-related genes. The SNP loci can be used to screen wheat with excellent drought tolerance characteristics, and play an important role in breeding wheat varieties resistant to grain filling stress.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a marker and application of a gene related to chlorophyll content in wheat. Background technique [0002] Wheat leaves senescence from bottom to top according to leaf age (Feller et al., 1986). Especially in the late growth period, drought stress will change the number, shape and content of chloroplasts, leading to premature termination of photosynthesis, affecting grain filling, and causing Substantial reductions in production (Troy et al., 2016). Among them, the flag leaf is the only leaf that transports nutrients to the grain throughout the whole process of fruiting, and plays a dominant role in the formation of yield. Although the second and third leaves play an important role in grain filling, they all decline before the flag leaf. The period from flowering to milk maturity is the main period for the formation of grain weight. Due to the large amount of nutrients required by t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 肖永贵杨梦娇武玉莹何中虎夏先春
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI