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Manual syringes for cell manipulation

A technology of syringes and cells, which is applied in the direction of instruments, biochemical instruments, stress-stimulated microbial growth methods, etc., and can solve problems such as pollution and incompatibility

Active Publication Date: 2022-03-15
EPPENDIRF AG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A disadvantage, however, is that hydraulic syringes must be filled with liquid without air bubbles and must be refilled after a certain number of operations
Furthermore there is a risk of contamination and incompatibility through contact between liquid and cells

Method used

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  • Manual syringes for cell manipulation
  • Manual syringes for cell manipulation
  • Manual syringes for cell manipulation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0049] In this application, the statements "vertical" and "horizontal", "above" and "below" and expressions derived therefrom such as "above" and "below", "upper side" and "below" refer to Orientation of the displacement device in which the center axes of the base body and the rotating wheel are aligned vertically and the rotating wheel is arranged at least partially above the base body.

[0050] The syringe 1 has a displacement device 2 , an injection tube 3 and a capillary holder 4 in which a microcapillary 5 is held (this is not shown).

[0051] The displacement device 2 has a disc-shaped base body 6 which, on a first end side 7 , has a hollow-cylindrical cylinder space 9 concentric to the central axis 8 . The cylinder space 9 has a cylindrical inner wall 10 and a cylindrical outer wall 11 as well as a circular disk-shaped bottom wall 12 .

[0052] On the first end side 7 , the cylinder space 9 has a circular cylinder opening 13 through which it is accessible from the firs...

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PUM

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Abstract

The invention relates to a manual syringe for cell manipulation, the syringe has a displacement device for displacing fluid, an injection hose connected to the displacement device and a capillary holder connected to the injection hose for holding micro Capillary or microcapillary connected to the injection tube, wherein the displacement device has the following features: * a disc-shaped base body, * a disc-shaped rotating wheel arranged on the first end side concentrically with the base body, * Screw drive with a rod nut and a threaded rod engaged therein and arranged on the central axis of the base body, *arranged in the cylinder space, on the inner circumference of the cylinder space on the inner wall and on the outer circumference A hollow-cylindrical piston that rests sealingly on the outer wall of the cylinder space, * a rotary decoupling and * a connection to the cylinder space, to which the injection hose is connected.

Description

technical field [0001] The invention relates to a manual syringe for cell manipulation as well as a method for cell manipulation and the use of the manual syringe. Background technique [0002] Typical tasks in cell manipulation are maintaining cells, injecting solutions, cells and cellular components in cells or tissues and removing or admitting cells, cellular components or solutions. A syringe (microinjector) is used for this. The syringe has a displacement device for the fluid, which is connected via a hose to a capillary holder for holding a replaceable microcapillary. Manual syringes have a manually actuated displacement mechanism. Microcapillaries are needle tubes with a tip having an inner diameter in the range of 1 to 200 μm, preferably 4 to 100 μm. Preferably the microcapillary is made of glass. [0003] A vacuum or overpressure is generated in the microcapillary by means of a displacement device in order to retain, remove or inject cells, cell components or so...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/42
CPCC12M35/00C12M23/50G02B21/32C12M33/04
Inventor C·弗鲁克
Owner EPPENDIRF AG