A Comparative Hybrid Population Sampling Method for Establishing Environmentally Safe Detection of Transgene Drift

An environmentally safe and transgenic technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problem of inconsistency in genetic background, inability to truly and accurately reflect fitness effect, and inconsistency in genetic background of parental allele partial segregation. And other issues

Active Publication Date: 2021-04-30
FUDAN UNIV
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Problems solved by technology

However, this traditional sampling method can lead to differences in the partial segregation of parental alleles and genetic background inconsistencies between the two comparison populations, that is, the population containing the transgene has more crop alleles, while the Populations without the transgene have more CWR alleles
In addition to the difference in the presence or absence of genetically modified genes, the two groups are also inconsistent in genetic background, which leads to the fact that the results of fitness evaluation cannot truly and accurately reflect the fitness effect brought about by genetically modified genes.

Method used

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  • A Comparative Hybrid Population Sampling Method for Establishing Environmentally Safe Detection of Transgene Drift
  • A Comparative Hybrid Population Sampling Method for Establishing Environmentally Safe Detection of Transgene Drift
  • A Comparative Hybrid Population Sampling Method for Establishing Environmentally Safe Detection of Transgene Drift

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Experimental program
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Embodiment 1

[0017] Insect-resistant transgenes constructed using traditional sampling methods CpTI (Positive population, P+) and no insect-resistant transgene CpTI (Negative population, P-) comparative experimental population, in the process of genetic analysis of the P+ population and P- population, it was found that the P+ and P- comparison populations showed the frequency of alleles from their parents at some SSR loci Different, with partial segregation phenomenon, further calculate the genetic differentiation index between P+ and P-comparison groups ( F ST ) values ​​indicate that there are significant differences in the genetic background between the comparison groups ( figure 1 ), such differences will seriously affect the resistance to insect transgenics CpTI Results of the analysis of the fitness effect.

[0018] Constructs containing insect-resistant transgenes using traditional sampling methods CpTI (Positive population, P+) and no insect-resistant transgene CpTI (Neg...

Embodiment 2

[0038] Using the sampling method of the present invention to construct insect-resistant transgenes CpTI (Positive population, P+) and no insect-resistant transgene CpTI (Negative population, P-) compared to the experimental population. During the genetic analysis of the P+ population and the P- population, it was found that at some SSR loci, the P+ and P-comparison populations showed partial segregation of the alleles from the parents, but the P+ population and the P-comparison population The genetic differentiation index between ( F ST ) values ​​are very small, indicating that the genetic background between the P+ population and the P- population is very close ( figure 2 ), the detected fitness difference can truly reflect the difference brought about by the transgene, which is beneficial to the CpTI Proper assessment of the environmental risk of insect-resistant transgene drift into crop wild relatives.

[0039] Using the sampling method of the present invention to...

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Abstract

The invention belongs to the technical field of environmental risk assessment of genetically modified organisms. Specifically, it is a comparative hybrid population sampling method for establishing environmental safety detection of transgene drift. The method of the present invention utilizes a transgene from a crop and a neutral molecular marker that is from the same chromosome of the crop but is very close to the transgene and has no physical linkage for sampling, and respectively constructs hybrid offspring segregation groups that contain the transgene or do not contain the transgene, Make the genetic backgrounds of these two populations infinitely close, and then analyze and compare the fitness-related traits of the two populations under the environmental conditions of homogenous gardens, so as to accurately predict and evaluate the transgenes that enter the wild relatives of crops through natural hybridization Will it cause environmental risks. The method of the invention greatly reduces the difference of allelic segregation and the inconsistency of genetic backgrounds between the comparison populations (P+ and P-) caused by sampling, and makes the result of the safety evaluation of genetically modified organisms closer to the real situation.

Description

technical field [0001] The invention belongs to the technical field of environmental risk assessment and analysis of genetically modified organisms, and in particular relates to a comparative hybrid population sampling method for establishing environmental safety detection of transgenic drift. Background technique [0002] The rapid development of genetically modified technology has continuously promoted the cultivation of genetically modified plants. The commercial planting of genetically modified crops has provided new ways to increase crop yields, reduce pesticide use, improve the agricultural ecological environment, and solve food security problems. However, transgenes may escape from crops to their wild relative populations through gene drift, which may pose certain environmental risks. Therefore, before the commercialization of transgenic crops, the potential environmental risks that may be caused by transgenic drift must be detected and evaluated to ensure the safe an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/24
CPCC12Q1/24
Inventor 蔡星星卢宝荣王哲汪智
Owner FUDAN UNIV
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