A kind of culture liquid and culture method of hydroponic propagation of golden loin

A technology of cotton-haired golden waist and culture solution, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve problems such as shortage of golden waist resources, shorten rooting time, increase survival rate of transplanting, and promote reproduction Effect

Active Publication Date: 2020-03-27
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, my country's golden waist resources are in short supply, and domestic and foreign research on the hydroponic rapid propagation technology of golden waist, especially the hydroponic rapid propagation technology of cottony golden waist, can be said to be blank

Method used

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  • A kind of culture liquid and culture method of hydroponic propagation of golden loin
  • A kind of culture liquid and culture method of hydroponic propagation of golden loin
  • A kind of culture liquid and culture method of hydroponic propagation of golden loin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The hydroponic propagation medium of embodiment 1 golden waist explant

[0035] In order to explore the effect of different culture medium composition on the hydroponic propagation effect of the velvety golden loin explant, we designed an experiment with three change factors as the research object, one is the type of medium added, and the other is the level of hormone added. The third is the mass concentration of the added hormone. There are four types of culture medium used in the experiment, and each type has five types, of which A, C, and D are added with MS (weigh 4.43g MS per 1000mL of MS medium). powder), 1 / 2MS (weigh 2.47g1 / 2MS powder per 1000mL of 1 / 2MS medium), B5 (weigh 30.21g B5 powder per 1000mL of B5 medium) and hormones of different mass concentrations, while B Only hormones are added to the type 1 culture solution, and tap water is used as the solvent for the four types of culture solutions.

[0036] The preparation method of the culture medium (taking T...

Embodiment 2

[0041] Embodiment 2: the method for the hydroponic propagation of cotton wool golden waist explant

[0042] In order to improve the rigor of the culture results, in the culture process, 20 kinds of culture solutions were prepared in Example 1, and each culture solution was set up with 6 repetitions, wherein 2 explants were placed in culture dishes, and each culture dish consisted of One kind of culture medium was used to observe the culture effect of a single explant; the other 4 explants were cultured in a 100-grid freezer box so as to observe the overall culture effect and compare the culture effects of different culture fluids.

[0043] The specific method of the hydroponic propagation of the velvety golden waist explant is as follows:

[0044] 1. Selection of explants

[0045] There are several points for attention in the selection of explants: one is to have a targeted and purposeful selection; the other is to observe the overall growth state of the plant, and set the ta...

Embodiment 3

[0055] Embodiment 3: The influence of different culture fluids on overall culture effect

[0056] During the culture of the explants, their growth status was observed every day, and photographed and recorded. pass Figure 1-4 The results shown show that the growth of the explants in the three types of culture medium A, C, and D is poor, and the leaves become wilted or scorched around the fifth day of cultivation, and the area expands with time until the explants The petiole becomes soft. Wherein, the leaves of the explants in the type A culture medium will turn red after wilting. The explants in the culture mediums of C and D are the same as A, and the leaves of the explants start to wilt from the edge and the area expands with time. The explants in the culture medium, and A, C, D three types of culture medium 6 groups repeated experiment results are the same. However, the explants in the Type B culture medium have been growing well and kept fresh and tender, and the resul...

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Abstract

The invention relates to a culture solution for hydroponic propagation of Chrysosplenium lanuginosum and a water culture method. According to the invention, young and tender stem segments of Chrysosplenium lanuginosum are adopted as the explant for culture with different culture solutions, and according to whether the explant can generate adventitious root and axillary bud and the length of time needed, a culture solution suitable for hydroponic propagation of Chrysosplenium lanuginosum can be selected and transplanted to the outdoor. The culture method provided by the invention can achieve ahigh transplantation survival rate, greatly promotes the propagation of Chrysosplenium lanuginosum, effectively shortens the rooting time, can provide the raw material source for large-scale plantingand field management of Chrysosplenium lanuginosum, and is suitable for large-scale industrial production.

Description

technical field [0001] The invention belongs to the technical field of hydroponic propagation of plants, and in particular relates to a culture solution and a cultivation method for the hydroponic propagation of golden waist. Background technique [0002] Chrysosplenium is a plant belonging to Saxifragaceae (Saxifragaceae). There are about 65 species in the world. They are distributed in Asia, Europe, Africa, and America, but they are mainly produced in the temperate zone of East Asia. Generally, it is divided into Subg.Gamosplenium and Subg.Chrysosplenium or Subg.Dialysplenium according to the opposite or alternate leaves: there are about 33 species of Subg.Gamosplenium in the world, and 23 species and 6 varieties are produced in China; There are about 32 species in the genus Lumosa in the world, 11 species and 2 varieties in China; there are about 51 species in East Asia, and 36 species and 8 varieties in China, both in the north and the south, and 21 of them are endemic t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
CPCA01H4/001A01H4/008
Inventor 张丽刘虹覃瑞刘秤利张丹丹李刚
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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