Antineoplastic purpose of taro leaf polysaccharide

An anti-tumor and bean taro technology, applied in the field of medicine, can solve the problems of few reports on anti-tumor activity, achieve significant anti-tumor activity, reduce tumor weight, and expand the application field

Inactive Publication Date: 2018-11-16
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, many nutrition and health food researchers are very concerned about the research and topi

Method used

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  • Antineoplastic purpose of taro leaf polysaccharide
  • Antineoplastic purpose of taro leaf polysaccharide
  • Antineoplastic purpose of taro leaf polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Embodiment 1, the antitumor application of bean taro leaf polysaccharide, such as Figure 1-Figure 8 As shown, bean taro leaf polysaccharide has a significant anti-tumor effect. The specific preparation steps of bean taro leaf polysaccharide are as follows:

[0049] (1), preparation of bean taro leaf polysaccharide crude product:

[0050] (1.1), according to the solid-liquid ratio of 1g:10ml, weigh 100g of taro leaves and add 1000mL of distilled water, extract in 80°C water bath for 4h (enzyme inactivation is also achieved during extraction), then beat, and then centrifuge (4000r / min centrifuge 30min), the supernatant obtained by centrifugation was suction filtered and the filtrate was collected;

[0051] (1.2), replace the bean taro leaves in step (1.1) with the centrifuged filter residue obtained in step (1.1), repeat the extraction 2 times, combine the filtrate three times and concentrate by rotary evaporation until it is 1 / 5 of the original volume to obtain a conc...

Embodiment 2

[0067] Embodiment 2, the effect of bean taro leaf polysaccharide on tumor volume in mice;

[0068] The mouse tumor volume (before putting to death) after intragastric gavage in the measurement embodiment 1 28 days, the result is as follows figure 2 As shown, the tumor volume in the control group was the largest. Compared with the control group, the positive control group and the intervention group with different concentrations of bean taro leaf polysaccharide had a certain inhibitory effect on the increase of tumor volume, and the tumor volume in the positive drug group was the smallest. The above results indicated that the taro leaf polysaccharide can alleviate the increase of tumor volume in tumor-bearing nude mice.

Embodiment 3

[0069] Embodiment 3, the influence of bean taro leaf polysaccharide on mouse tumor weight and tumor inhibition rate;

[0070] After 28 days of intragastric administration in Example 1, the mice were fasted for 12 hours, and the mice were killed by cervical dislocation after blood was collected from the orbits. According to the formula: tumor inhibition rate=(1-weight of tumor mass in experimental group / weight of tumor mass in control group)*100%. The result is as image 3 , Figure 4 It was shown that the positive control group and the intervention group of bean taro leaf polysaccharide with different concentrations all had inhibitory effect on tumor growth, and the tumor inhibition rate of the positive drug group was the highest.

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Abstract

The invention discloses an antineoplastic purpose of taro leaf polysaccharide. According to the invention, Liver cancer cells Huh7 are inoculated in subcutaneous positions of nude mice to form tumor;at the same time, the taro leaf polysaccharide is subjected to intervention; and the body weight change condition of the nude mice in the experiment process is monitored. After the intervention experiment is completed, the subcutaneous tumor is peeled; the tumor weight is recorded; the cell factor secretion condition in the serum is monitored; and the taro leaf polysaccharide has obvious anti-tumor effect.

Description

technical field [0001] The invention relates to the field of medicine, in particular to an antitumor application of bean taro leaf polysaccharide. Background technique [0002] Liver disease, in modern medicine, refers to diseases of the liver organ based on anatomical morphology, such as viral acute, chronic hepatitis, cirrhosis, schistosomal liver disease, fatty liver, liver cysts, and hepatic cavernous hemangiomas. The main cause of liver disease is hepatitis B virus infection, and the excessive compensatory proliferation of liver cells caused by long-term chronic inflammation can induce liver cancer. About 2 billion people in the world have been infected with hepatitis B, and 30% of the 350 million chronic hepatitis B virus carriers develop into liver cirrhosis; the number of people infected with hepatitis B virus in my country reaches 120 million, and about 20% of patients with chronic hepatitis develop into liver cirrhosis and liver cancer. %-90% of liver cancer patien...

Claims

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Application Information

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IPC IPC(8): A61K31/715A61P35/00A23L33/125C08B37/00
CPCA61K31/715A23L33/125A23V2002/00A61P35/00C08B37/0003A23V2200/308A23V2200/332A23V2250/51
Inventor 郑晓冬楚强刘阳阳俞露霜李永璐陈鹏华
Owner ZHEJIANG UNIV
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