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Preparation and extraction method for umbilical cord blood stem cell

A technology of umbilical cord blood stem cells and extraction methods, applied in cell dissociation methods, biochemical equipment and methods, animal cells, etc., can solve the problems of not being able to guarantee the health of stem cells and the complicated method of removing impurities from umbilical cord blood, and achieve simplified removal , cost reduction effect

Inactive Publication Date: 2018-11-16
安徽
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the deficiencies of the prior art, the present invention provides a method for preparing and extracting umbilical cord blood stem cells, which solves the problem that the existing stem cell extraction methods cannot guarantee the health of stem cells and the method for removing impurities in umbilical cord blood is relatively complicated

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  • Preparation and extraction method for umbilical cord blood stem cell

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Comparison scheme
Effect test

Embodiment 1

[0026] A method for preparing and extracting umbilical cord blood stem cells, characterized in that: comprising the following steps,

[0027] (1) Save the backup, extract the cord blood and store it in the freezer;

[0028] (2) Stem cell extraction, take part of the cord blood with the corresponding label and heat it in a water bath, slowly thaw the frozen cord blood, extract plasma from the cord blood, and use a blood separator to extract cord blood stem cells;

[0029] (3) Health test, the umbilical cord blood stem cells were inoculated in a medium containing inducing factors for culture, and the cell activity was detected after 2 days of induction and differentiation;

[0030] (4) Screening, the umbilical cord blood with poor cell activity after induction of differentiation was taken out of the freezer and discarded, and the umbilical cord blood with good cell activity was continued to be stored in the freezer.

[0031] Cord blood should be frozen and isolated in a freezer...

Embodiment 2

[0041] A method for preparing and extracting umbilical cord blood stem cells, characterized in that: comprising the following steps,

[0042] (1) Save the backup, extract the cord blood and store it in the freezer;

[0043] (2) Stem cell extraction, take part of the cord blood with the corresponding label and heat it in a water bath, slowly thaw the frozen cord blood, extract plasma from the cord blood, and use a blood separator to extract cord blood stem cells;

[0044] (3) Health test, the umbilical cord blood stem cells were inoculated in a medium containing inducing factors for culture, and the cell activity was detected after 2 days of induction and differentiation;

[0045] (4) Screening, the umbilical cord blood with poor cell activity after induction of differentiation was taken out of the freezer and discarded, and the umbilical cord blood with good cell activity was continued to be stored in the freezer.

[0046] Cord blood should be frozen and isolated in a freezer...

Embodiment 3

[0056] A method for preparing and extracting umbilical cord blood stem cells, characterized in that: comprising the following steps,

[0057] (1) Save the backup, extract the cord blood and store it in the freezer;

[0058] (2) Stem cell extraction, take part of the cord blood with the corresponding label and heat it in a water bath, slowly thaw the frozen cord blood, extract plasma from the cord blood, and use a blood separator to extract cord blood stem cells;

[0059] (3) Health test, the umbilical cord blood stem cells were inoculated in a medium containing inducing factors for culture, and the cell activity was detected after 2 days of induction and differentiation;

[0060] (4) Screening, the umbilical cord blood with poor cell activity after induction of differentiation was taken out of the freezer and discarded, and the umbilical cord blood with good cell activity was continued to be stored in the freezer.

[0061] Cord blood should be frozen and isolated in a freezer...

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Abstract

The invention discloses a preparation and extraction method for an umbilical cord blood stem cell. The preparation and extraction method comprises the following steps:preserving backup; extraction thestem cell; taking partial umbilical cord blood with a corresponding marking number and heating in a water bath; slowly unfreezing the frozen umbilical cord blood, extracting blood plasma from the umbilical cord blood, and extracting the umbilical cord blood stem cell by using a blood separating machine; detecting the health, inoculating the umbilical cord blood stem cell into a culture medium containing an inducing factor for culturing, carrying out induced differentiation for 2 days and then detecting cell activity; carrying out health detection; screening, and taking out the umbilical cordblood which is subjected to the induced differentiation and has poorer cell activity from a freezing chamber for discarding; continuously putting the umbilical cord blood with good cell activity intothe freezing chamber for preserving. The invention relates to the technical field of bioengineering. The preparation and extraction method for the umbilical cord blood stem cell has the advantages that the umbilical cord blood stem cell with poorer health conditions can be effectively removed, and the problems caused by gene deletion of the umbilical cord blood is reduced; meanwhile, the process of removing impurities in the umbilical cord blood is simplified, and the cost is reduced.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a method for preparing and extracting umbilical cord blood stem cells. Background technique [0002] Stem cells are a kind of pluripotent cells with self-replication ability. Under certain conditions, they can differentiate into a variety of functional cells. According to the developmental stage of stem cells, they can be divided into embryonic stem cells and adult stem cells. According to the developmental potential of stem cells, they can be divided into three types: Classes: totipotent stem cells, pluripotent stem cells and unipotent stem cells (multipotent stem cells), stem cells are not fully differentiated, immature cells with the potential to regenerate various tissues and organs and the human body. with cells". However, the existing stem cell extraction methods cannot guarantee the health of stem cells, and the method of removing impurities in cord blood is relati...

Claims

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Application Information

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IPC IPC(8): C12N5/0775
CPCC12N5/0665C12N2501/15C12N2501/231C12N2509/00
Inventor 安徽
Owner 安徽