A kit for detecting rnf213 mutant gene in plasma free DNA and its application
A mutated gene and kit technology, applied in the fields of biotechnology and medicine, can solve the problems of difficult determination and high cost of detection
Active Publication Date: 2019-05-10
THE SECOND HOSPITAL OF SHANDONG UNIV
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Problems solved by technology
High-throughput sequencing is an efficient method for studying ctDNA at present, but the cost of detection is high, and it is difficult to determine whether the mutated gene found in the detection is related to lung cancer
Method used
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Embodiment Construction
[0012] Technical scheme of the present invention is as follows:
[0013] RNF213 mutant genes in plasma cell-free DNA, including the following mutant genes and mutation sites: NM_001256071:exon26:c.G5960A:p.G1987E, NM_001256071:exon59:c.C14226A:p.S4742R, NM_001256071:exon29:c.A8252T:p .N2751I, NM_001256071:exon24:c.G4615A:p.A1539T, NM_001256071:exon26:c.C5171T:p.A1724V, NM_020954:exon17:c.A3101T:p.K1034M.
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Abstract
The invention relates to a kit for detecting a RNF213 mutant gene in free DNA of blood plasma and applications thereof. The invention discloses a RNF213 mutant gene in free DNA of blood plasma and mutation sites thereof, and a kit for detecting the RNF213 mutant gene. The kit comprises specific primers, specific probes, a buffer solution, dNTPs, TaqDNA polymerase, MgCl2, and a DNA extraction kit;wherein the specific primers and specific probes are designed based on the RNF213 gene, and the nucleotide sequences of the specific primers and specific probes are represented by SEQ ID No.1-24. Forthe first time, the application of RNF213 gene mutation in non-small cell lung cancer diagnosis is disclosed, and the malignant tumor and benign tumor can be well distinguished. The provided kit can be used to detect RNF213 gene mutation and provides guidance for the diagnosis of pulmonary nodule.
Description
technical field [0001] The invention relates to a kit for detecting RNF213 mutation gene in plasma free DNA and application thereof, belonging to the fields of biotechnology and medicine. Background technique [0002] Lung cancer is currently the malignant tumor with the highest morbidity and mortality in the world. According to statistics, among the lung cancer patients who go to hospitals in China and receive treatment, early lung cancer (stage I) only accounts for 19.10%, that is to say, 80% The above lung cancer patients are in the middle and late stages when they go to the hospital for treatment. The 5-year survival rate of patients with early-stage lung cancer is over 90%, and the 10-year survival rate is close to 70%. The 5-year survival rates of patients with intermediate and advanced lung cancer are about 30% and 10%, respectively. Therefore, the effective way to improve the cure rate of lung cancer is: early detection, early diagnosis and early treatment. [000...
Claims
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IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/156
Inventor 赵小刚姜宁毛海婷孙启峰李培超
Owner THE SECOND HOSPITAL OF SHANDONG UNIV