Intrinsic reference gene and its application for fluorescence quantitative analysis of wild hemp and cultivated hemp

Abstract

The invention discloses an internal reference gene for the fluorescence quantitative analysis of wild cannabis and cultivated cannabis and its application. The internal reference gene is the gene PP2A or the gene EF1α, and the expression stability of the gene PP2A or the gene EF1α is the highest in different parts of wild cannabis and cultivated cannabis at the beginning of fruiting stage, which solves the current situation that there is no internal reference gene in the gene expression research of wild cannabis and cultivated cannabis , which provides strong support for the next step in the study of gene expression in different genotypes of cannabis.

Description

technical field [0001] The invention relates to the field of plant molecular biology, in particular to an internal reference gene used for fluorescent quantitative analysis of wild cannabis and cultivated cannabis and its application. Background technique [0002] Hemp (Cannabis sativa L.), also known as hemp, thread hemp, is an annual herb of Cannabinaceae (Cannabinaceae) genus (Cannabis). Food and pharmaceutical fields. Hemp mainly originated in Central Asia and is now distributed worldwide (cultivated or wild). my country is rich in cannabis resources, of which wild cannabis is distributed in Yunnan, Tibet, Xinjiang, Inner Mongolia and Northeast China, while cultivated cannabis is distributed more widely, covering most provinces and regions in my country, currently mainly concentrated in Yunnan, Heilongjiang, Inner Mongolia, Gansu, Shanxi and Anhui. Wild and cultivated hemp showed significant differences in biological and agronomic traits such as plant height, branch nu...

AI Technical Summary

Problems solved by technology

However, a large number of research results in recent years have shown that there is no internal reference gene with absolutely stable expression, and the so-called constant expression of any internal reference gene is only a "range" constant expression under certain types of cells or experimental conditions.

So far, there have been many reports on the screening and verification of reference genes in crops such as rice, corn, potato, cucumber, tomato, and apple, but there is no report on the expression analysis of genes related to cannabinoid synthesis in wild cannabis and cultivated cannabis. Appropriate internal reference genes are available

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