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Applications of long non-coding RNAs

A 1.CCAT2, cervical cancer technology, applied in the application field of long-chain non-coding RNAs, can solve the problems of large radiation dose, unsuitable for early screening of cervical cancer, and high cost of imaging examinations

Active Publication Date: 2021-09-10
AFFILIATED HOSPITAL OF ZUNYI UNIV +1
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

However, the cost of imaging examination is high, and the radiation dose is relatively large, so it is not suitable for early screening of cervical cancer.

Method used

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  • Applications of long non-coding RNAs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Example 1: Detection of the level of serum non-coding RNA by real-time quantitative PCR

[0053] Serum samples were collected from 115 patients with cervical squamous cell carcinoma (CESC) and 101 normal controls, all of whom were of Han nationality and had no direct relatives. Patients with cervical squamous cell carcinoma were treated at the Cancer Hospital of the Chinese Academy of Medical Sciences from 2016 to 2017. The inclusion criteria of patients were based on pathological examination. All samples were confirmed by histopathology or cytology, without radiotherapy, chemotherapy and surgery before blood collection; patients with previous history of other tumors were excluded. Normal controls were randomly selected from the normal population who underwent physical examination in the Cancer Hospital of Chinese Academy of Medical Sciences during the same period. The inclusion criteria for the normal control group were: no tumor history and clinical signs, gender an...

Embodiment 2

[0056] Example 2: ROC analysis of 3 long non-coding RNAs as CESC serum markers

[0057] According to the results of real-time quantitative PCR in the serum of patients with cervical squamous cell carcinoma and normal people, the corresponding ROC curve was calculated according to -△△ct, and the sensitivity and sensitivity were determined by observing the area under the curve and the range of 95% confidence interval. Specificity; the value of the area under the ROC curve is between 1.0 and 0.5. In the case of AUC>0.5, the closer the AUC is to 1, the better the diagnostic effect. When AUC is 0.5-0.7, it has low accuracy; when AUC is 0.7-0.9, it has certain accuracy; when AUC is above 0.9, it has high accuracy; when AUC=0.5, it means that the diagnostic method does not work at all and has no diagnostic value. ; AUC<0.5 does not conform to the real situation, and rarely occurs in practice.

[0058] Such as figure 2 As shown, the AUC values ​​of CCAT2, LINC00511, and LINC01133 w...

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Abstract

The invention relates to the field of biotechnology, and discloses new applications of CCAT2, LINC00511 and LINC01133 among long-chain non-coding RNAs in the field of cervical cancer diagnosis. The present invention found that the expression of CCAT2, LINC00511 and LINC01133 in the serum of cervical cancer increased, and they are potential cervical cancer diagnostic markers. They are combined with squamous cell carcinoma antigen (SCC) as tumor markers for joint diagnosis, and have stronger specificity and sensitivity. Therefore, The present invention provides a new application of CCAT2, LINC00511 and LINC01133 and the combination of the three and SCC in the field of cervical cancer diagnosis, thereby providing a new diagnostic method for diseases in this field, and also supplementing the three of CCAT2, LINC00511 and LINC01133 Research results.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of long-chain non-coding RNAs. Background technique [0002] According to the data published by the National Cancer Center in CA: A Cancer Journal for Clinicians in 2016, cervical cancer is the second most common female malignancy in my country. In 2015, the number of new cases and deaths of cervical cancer in my country was 98,900 and 30,500, respectively. In the past 20 years, the morbidity and mortality in our country have been increasing year by year, and they are getting younger and younger. Previous studies have confirmed that persistent infection of high-risk human papillomavirus (Hr-HPV) is the main cause of cervical cancer. There are more than 100 recognized human papillomaviruses (HPV). 30-40 types of HPV are associated with external genital infection, 43 of which have cancer risk (ie Hr-HPV). The 14 confirmed Hr-HPVs are HPV16, 18, 31, 33, 35, 39, 45, 5...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/158C12Q2600/178
Inventor 罗清黄常志王文杰赵玫李燕
Owner AFFILIATED HOSPITAL OF ZUNYI UNIV
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