Application of TLCD1 to diagnosis, prognosis and treatment of BC (breast cancer) metastasis

A breast cancer and bone metastasis technology, applied in the field of biomedicine, can solve problems such as affecting the quality of life and longevity of patients, increasing the economic burden of patients' families, and difficult clinical treatment.

Active Publication Date: 2018-11-20
江门市中心医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In most cases, breast cancer has already caused bone metastases before causing obvious clinical symptoms, or the primary tumor has not recurred after treatment, but the formation of bone metastases is still found, which has brought great significance to clinical treatment. big difficulty
Once breast cancer has bone metastasis, it indicates that the patient has entered the advanced stage of the tumor, which seriously affects the quality of life and life expectancy of the patient, increases the economic burden of the patient's family, and consumes a lot of medical resources

Method used

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  • Application of TLCD1 to diagnosis, prognosis and treatment of BC (breast cancer) metastasis
  • Application of TLCD1 to diagnosis, prognosis and treatment of BC (breast cancer) metastasis
  • Application of TLCD1 to diagnosis, prognosis and treatment of BC (breast cancer) metastasis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Example 1, TLCD1 is highly expressed in bone metastasis tissues and cells of breast cancer

[0059] In the collected tissue samples and cell lines, the mRNA and protein levels of TLCD1 were detected by fluorescent quantitative PCR and Western blotting, and the results were as follows: figure 1 As shown, compared with breast cancer tissues without bone metastasis, the mRNA and protein levels of TLCD1 in breast cancer tissues with bone metastasis increased ( figure 1 A and figure 1 B), compared with human normal breast epithelial cell lines, the mRNA and protein content of TLCD1 in breast cancer cell lines increased, and the mRNA and protein content of TLCD1 increased significantly in metastatic breast cancer cell lines, especially MCF-7 and MDA-MB-231 cell line ( figure 1 C and figure 1 D).

[0060] The above results show that: TLCD1 is highly expressed in bone metastasis tissues and cells of breast cancer. Therefore, the occurrence of bone metastasis of breast cance...

Embodiment 2

[0061] Example 2. High expression of TLCD1 indicates short survival time without bone metastasis

[0062] 231 breast cancer tissue samples without bone metastasis were divided into TLCD1 high expression group and TLCD1 low expression group according to the median value of TLCD1 expression, and the bone metastasis-free survival time was further analyzed in combination with the follow-up data of breast cancer tissue samples. The results are as follows: figure 2 As shown, the bone metastasis-free survival time of the TLCD1 high expression group was longer.

[0063] The above results show that high expression of TLCD1 indicates a short bone metastasis-free survival time. Therefore, by detecting the expression level of TLCD1 and using the bone metastasis-free survival time as a prognostic indicator, it can be used to predict whether there is a tendency to bone metastasis of breast cancer; The expression level of TLCD1 mRNA or protein in the breast cancer tissue sample of the subje...

Embodiment 3

[0064] Example 3, downregulation of TLCD1 gene expression can inhibit breast cancer cell migration and invasion

[0065] MDA-MB-231 or MCF-7 cells were transfected with RNA interference plasmids and control plasmids capable of down-regulating TLCD1 gene expression, and the results were as follows image 3 As shown, the mRNA and protein expression levels of TLCD1 gene in MDA-MB-231 and MCF-7 cells transfected with RNA interference plasmid were significantly down-regulated.

[0066] The transfected MDA-MB-231 and MCF-7 cells were subjected to invasion and migration experiments, and the results Figure 4 As shown, the migration and invasion abilities of MDA-MB-231 and MCF-7 cells transfected with RNA interference plasmids were significantly inhibited.

[0067] The above results indicated that down-regulating the expression of TLCD1 gene can inhibit the migration and invasion of breast cancer cells.

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Abstract

The invention discloses an application of TLCD1 to diagnosis, prognosis and treatment of BC (breast cancer) metastasis. High expression of TLCD1 in tissue and cells with BC bone metastasis is discovered, which predicts shorter survival time without bone metastasis, and in-vitro and in-vivo experiments prove that down-regulated expression of the gene TLCD1 can inhibit BC migration and invasion, sothat BC bone metastasis is inhibited; based on the discoveries, a reagent for detecting TLCD1 gene expression level is provided to be used for preparing a kit for detecting whether BC metastasis occurs and/or a prognosis kit for predicting BC metastasis tendency; a TLCD1 inhibitory reagent is applied to preparation of drugs for preventing and/or treating BC metastasis, and a new idea is provided for diagnosis, prognosis and treatment of BC metastasis.

Description

technical field [0001] The invention belongs to the field of biomedicine, and more specifically relates to the application of TLCD1 in the diagnosis, prognosis and treatment of breast cancer metastasis. Background technique [0002] Metastasis is the main cause of death in cancer patients, and more than 90% of tumor-related deaths are related to distant organ metastasis. Bone is one of the most common distant metastatic organs of solid tumors. Bone-related complications, such as pain, pathological fractures, hypercalcemia, spinal cord compression, and loss of motor function, are accompanied by bone metastasis of tumors. eventually lead to the death of the patient. The current treatment for bone metastasis is palliative. Whether it is surgery, radiotherapy or drug treatment, it only relieves and improves the corresponding symptoms, and cannot significantly improve the survival expectancy of patients. Therefore, once bone metastasis occurs, it not only seriously affects the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886A61K45/00A61P35/04G01N33/574
CPCA61K45/00A61P35/04C12Q1/6886C12Q2600/158G01N33/57415G01N33/57484
Inventor 任栋李宇明杨春潇王斌张鑫
Owner 江门市中心医院
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