Immunosorbent for removing inflammatory factors in blood and preparation method thereof

Abstract

The invention discloses an immunosorbent for removing inflammatory factors in blood and a preparation method thereof. Based on a variety of intermolecular interactions between the antigenic epitope ofinflammatory factors such as IL-6, TNF-alpha, IL-1 beta and IL-8 and corresponding receptors thereof, affinity ligand of small molecule polypeptide with 5-18 selective amino acids is designed througha computer-assisted drug design method. Specifically, with vinyl acetate-triallyl isocyanurate as a basic skeleton, a mixture of ethyl acetate and alkane as a pore-forming agent, and a synthetic resin with benzoyl peroxide as an initiator as a carrier, alcoholysis activation is carried out, and then the small molecule polypeptide is grafted to obtain the small-molecule ligand immunosorbent whichdoes not adsorb macromolecular proteins and the like. The immunosorbent is simple to prepare, has high adsorbing capacity, high selectivity, good biological and blood compatibility, and relatively lowcost, and provides a new therapeutic method for removing excessive pathogenic inflammatory factors in patients.

Description

Technical field:

[0001] The invention belongs to the technical field of biomedicine. It specifically relates to a novel medical immunosorbent, especially an immunosorbent suitable for hemoperfusion to remove inflammatory factors in the body and a preparation method thereof.

[0002] technical background:

[0003] Inflammation is one of the most basic pathological changes. During the development of inflammation, it can stimulate the body's inflammatory cells (such as neutrophils, macrophages, endothelial cells, etc.) to release various cytokines, thereby causing systemic inflammatory reactions. Even cause sepsis and septic shock. More than 18 million people suffer from sepsis every year in the world. In our country, due to the abuse of antibiotics, poor air quality, water pollution, etc., there are also millions of sepsis patients every year, and the data show that the incidence of sepsis The fatality rate has surpassed myocardial infarction and has become the leading cause ...

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