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Immunosorbent for removing inflammatory factors in blood and preparation method thereof

An immunoadsorbent and inflammatory factor technology, applied in the field of biomedicine, can solve the problem of low selectivity, achieve high adsorption performance, low cost, and avoid non-specific adsorption effects

Active Publication Date: 2021-01-05
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these two adsorbents have achieved good adsorption effects in vitro, they mainly rely on hydrophobic interactions to remove toxins, which has the disadvantage of low selectivity, and may remove toxins while also removing beneficial substances in the body.

Method used

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  • Immunosorbent for removing inflammatory factors in blood and preparation method thereof
  • Immunosorbent for removing inflammatory factors in blood and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Preparation of polystyrene microspheres

[0025] Put monomer 53g vinyl acetate and crosslinking agent 10g triallyl isocyanurate into a 250mL beaker and mix evenly, then add 35.2g ethyl acetate, 35.9g n-heptane, 23.4g n-hexane Alkanes and 0.6g of benzoyl peroxide were stirred to dissolve them, and after the dissolution was complete, they were fully mixed to obtain an oil phase. Then add this system solution to 252mL of 2.8% polyvinyl alcohol solution (which contains 1.2% NaCl), adjust the stirring speed, and make the system disperse into uniform small oil droplets. After the temperature is raised to 52 ° C, after 5.5 hours of reaction, Then continue to heat up to 79 ° C for 3.5 hours, filter, the reaction system is filtered, washed with hot water and treated, then extracted with methanol for 16 hours to remove the porogen, dried in vacuum for 36 hours to obtain white copolymer microspheres, and then added to 1.2% In the NaOH / methanol solution, transesterification wa...

Embodiment 2

[0031] (1) Preparation of polystyrene microspheres

[0032] Put monomer 62.6g vinyl acetate and crosslinking agent 17.4g triallyl isocyanurate into a 500mL beaker and mix evenly, then add 84.5g ethyl acetate, 153.6g n-heptane, 145.9 g n-hexane and 4.64 g of benzoyl peroxide were stirred to dissolve them, and after the dissolution was complete, they were fully mixed to obtain an oil phase. Then add this system solution to 1299.2mL of 0.65% polyvinyl alcohol solution (which contains 9.6% NaCl), adjust the stirring speed, and make the system disperse into uniform small oil droplets. After the temperature is raised to 59 ° C, after 3.5 hours of reaction, Then continue to heat up to 72 ° C for 5 hours, filter, the reaction system is filtered, washed with hot water and treated, then extracted with methanol for 28 hours to remove the porogen, dried in vacuum for 24 hours to obtain white copolymer microspheres, and then added to 4.9% In NaOH / methanol solution, carry out transesterifi...

Embodiment 3

[0038] (1) Preparation of polystyrene microspheres

[0039] Put monomer 49.2g vinyl acetate and crosslinking agent 30.8g triallyl isocyanurate into a 500mL beaker and mix evenly, then add 29.6g ethyl acetate, 118.2g n-heptane, 92.2 1 g of n-hexane and 2.08 g of benzoyl peroxide were stirred to dissolve them, and after the dissolution was complete, they were fully mixed to obtain an oil phase. Then add this system solution to 740.8mL of 1.7% polyvinyl alcohol solution (containing 5.2% NaCl), adjust the stirring rate, and make the system disperse into uniform small oil droplets. After the temperature is raised to 55°C, react for 4.5h Then continue to heat up to 76 ° C for 4.5 hours, filter, the reaction system is filtered, washed with hot water and treated, then extracted with methanol for 36 hours to remove the porogen, dried in vacuum for 12 hours to obtain white copolymer microspheres, and then added to 2.6% In a NaOH / methanol solution, carry out transesterification at 50°C ...

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Abstract

The invention discloses an immunosorbent for removing inflammatory factors in blood and a preparation method thereof. Based on a variety of intermolecular interactions between the antigenic epitope ofinflammatory factors such as IL-6, TNF-alpha, IL-1 beta and IL-8 and corresponding receptors thereof, affinity ligand of small molecule polypeptide with 5-18 selective amino acids is designed througha computer-assisted drug design method. Specifically, with vinyl acetate-triallyl isocyanurate as a basic skeleton, a mixture of ethyl acetate and alkane as a pore-forming agent, and a synthetic resin with benzoyl peroxide as an initiator as a carrier, alcoholysis activation is carried out, and then the small molecule polypeptide is grafted to obtain the small-molecule ligand immunosorbent whichdoes not adsorb macromolecular proteins and the like. The immunosorbent is simple to prepare, has high adsorbing capacity, high selectivity, good biological and blood compatibility, and relatively lowcost, and provides a new therapeutic method for removing excessive pathogenic inflammatory factors in patients.

Description

Technical field: [0001] The invention belongs to the technical field of biomedicine. It specifically relates to a novel medical immunosorbent, especially an immunosorbent suitable for hemoperfusion to remove inflammatory factors in the body and a preparation method thereof. [0002] technical background: [0003] Inflammation is one of the most basic pathological changes. During the development of inflammation, it can stimulate the body's inflammatory cells (such as neutrophils, macrophages, endothelial cells, etc.) to release various cytokines, thereby causing systemic inflammatory reactions. Even cause sepsis and septic shock. More than 18 million people suffer from sepsis every year in the world. In our country, due to the abuse of antibiotics, poor air quality, water pollution, etc., there are also millions of sepsis patients every year, and the data show that the incidence of sepsis The fatality rate has surpassed myocardial infarction and has become the leading cause ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J20/26B01J20/30A61M1/36
CPCA61M1/3687A61M2202/0413B01J20/267A61M2202/0021
Inventor 欧来良柴雅敏
Owner NANKAI UNIV
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