Non-contact magnetic transmitting array type PCR micro-channel structure and amplification method

Abstract

The invention discloses a non-contact magnetic transmitting array type PCR micro-channel structure and an amplification method. The non-contact magnetic transmitting array type PCR micro-channel structure comprises three constant-temperature regions, namely a 96-DEG C region, a 72-DEG C region and a 55-DEG C region, and further comprises a coil group I, a coil group II, a coil group III and a coilgroup IV, which consist of excitation coils, wherein the excitation coils are uniformly wound on a transparent quartz guide tube; the transparent quartz guide tube is connected among the three constant-temperature regions; a PCR reagent module for performing amplification operation in the transparent quartz guide tube is formed by connecting a permanent magnet and a reagent cavity. A reagent pushing way disclosed by the invention is a reagent drive way without substance transmitting in a transmitting channel, so that a fault rate is greatly reduced. The three regions are unchanged constant-temperature regions, so that stable temperature control on PCR amplification is favorably realized. The transmitting mechanism is simple in structure, is convenient for an array type design, and is beneficial for function integration and structure reduction for space application.

Description

technical field [0001] The invention relates to a non-contact magnetic transmission array microchannel PCR amplification system, which belongs to the fields of biology, analytical chemistry and medical detection, in particular to a non-contact magnetic transmission array PCR microchannel structure design and PCR amplification control method . Background technique [0002] Since 1999, China's manned spaceflight project has been officially implemented, gradually realizing a series of major breakthroughs such as unmanned to manned flight, single-person single-day to multi-person multi-day. With the advancement of the third step of the three steps of aerospace engineering - the construction of the space station, the health of the astronauts is an important guarantee for the smooth completion of the work of the space station. Since the space station is in a closed state for a long time during orbital operation, the microorganisms in the space station will damage the health of as...

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Problems solved by technology

[0006] The purpose of the present invention is to propose a design of a non-contact magnetic transfer array PCR microchannel structure and a method for controlling PCR amplification in order to solve the problems of slow expansion speed, large volume and contact transmission mechanism of the current PCR amplification transmission mechanism. It is used for the amplification of PCR under the condition of weightlessness, and the miniature design of the PCR fluorescence instrument in the later stage

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